Purification, Analysis of Structure of a Polysaccharide From the Fruit of Lycium barbarum L.

Abstract:

A polysaccharide, coded as LBP5a, was extracted from the Lycium barbarum L. with distilled water. LBP5a was precipitated repeated by ethanol and purified by DEAE-52, Sephadex G-75. The structure of LBP5a was detected by FT-IR spectrometer, Ultraviolet spectrophotometer, experiments of periodate oxidation and Smith degradation. The results show that LBP5a is testified to be homogeneous polysaccharide, and does not contain nucleic acid or protein. Pyranose with 1→3, 1→4 glycosyl linkages mainly exist in LBP5a.

• Periodical: Advanced Materials Research (Volumes 550-553)
• Pages: 1719-1723
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.550-553.1719
• Online since: July 2012
• Authors: Min Zhang, Qian Ma, Jian Wang
• Keywords: Lycium barbarum L., Polysaccharide, Purification, Structure

References

[1] Y. P.Liu , H. Q.Mao , P.Li,: he effect of Lycium barbarum polysaccharide on T lymphocyte subset and lymphocyte transformation in mice. Journal of Qinghai Medical College, 21 4 (2000), pp.4-5, in Chinese.

[2] H.Ni, D. G.Qing, S.Kaisa and J. F. Lu: The study on the effect of LBP on cleaning hydroxygen free radical by EPR technique. Journal of Chinese Medicinal Materials, 27 8 (2004), pp.599-600.

[3] A. P.Vanita, R.L. Voctor, E. F.Karen, N. R.Venkat, and J. G. Frank: The Effects of Hyperbaric Oxygen on the Crystallins of Cultured Rabbit Lenses: a Possible Catalytic Role for Copper. Journal of Experimental Eye Research,71 4(2000),pp.373-383.

DOI: 10.1006/exer.2000.0887

[4] T. Nabekura, Y. Koizumi, M. Nakao,M. Tomohiro, M.Inomata and Y. Ito: Delay of cataract development in hereditary cataract UPL rats by disulfiram and aminoguanidine. Journal of Qinghai Medical College, 76 2 (2003), pp.169-174.

DOI: 10.1016/s0014-4835(02)00284-1

[5] J.J. Harding: Can drugs or micronutrients prevent cataract. Journal of Drugs Aging, 7 (2001), pp.473-486.

DOI: 10.2165/00002512-200118070-00001

[6] C. G. Kumar, H. S. Joo, R Kavali, J W Choi and C S Chang: Purification and characterization of extracellular polysaccharide from haloalkalophilic Bacillussp. Journal of Mathematical Medicine, 2 (2006), pp.130-131.

[7] G.J. Sun, Y.Zhang, J.S. Huang, S.K. Wang, Z.L. Sun and P.L. Huang: Study on the protective effect of Lycium barbarum polysaccharides on kidney and its mechanism in type Ⅱ diabetic rats. Journal of Acta Nutrimenta Sinica,1(2006), pp.101-103, in Chinese.

[8] H. Amagase , B. X. Sun, C.Borek, :Lycium barbarum juice improves in vivo antioxidant biomarkers in serum of healthy adults. Journal of Nutrition Research, 29 (2009), pp.19-25.

DOI: 10.1016/j.nutres.2008.11.005

[9] Q.Luo, Y. Z. Cai, J.Yan , M.Sun and H.Corke: Hypoglycemic and ypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Journal of Life Sciences, 76 (2004), pp.137-149.

DOI: 10.1016/j.lfs.2004.04.056

[10] L. M. Wang, W. S. Xia: Isolation and analysis of a novel acidic polysaccharide with glucokinase-stimulating activity from coarse green tea. Journal of Food Biochemistry, 30 (2006), pp.187-202.

DOI: 10.1111/j.1745-4514.2006.00052.x

[11] M. Zhang: Studied on Characteristic, structure and bioactivities of Lycium barbarum polysaccharide. (2003) , in Chinese.

[12] A. M.Staub: Removal of protein-Sevag method. Methods in Carbohydrate Chemistry. Journal of Methods in Carbohydrate Chemistry, 5 (1965), pp.5-6.

[13] J. H.Lee, J. S.Shim, J.S. Lee, J.K. Kim, I.S. Yang, M.S. Chung and K.H. Kim: Inhibition of pathogenic bacterial adhesion by acidic polysaccharide from green tea (Camellia sinensis). Journal of Agricultural and Food Chemistry, 54 (2006), pp.8717-8723.

DOI: 10.1021/jf061603i

[14] J.Chen, L.Zhang, D.Yu and R.Zhu: The chemical structure and solution properties of polysaccharides from Ganoderma lucidum mycelium. Journal of Chemical Journal of Chinese Universities, 21(2000), pp.961-964 , in Chinese.

[15] M.Zhao, Y.Chang, P. X.Wang, L.Zhang and O.Y. Zhen:Separation, purification and preliminarystructure analysis of polysaccharide PMP12 from Mulberry leaves. Journal of Jiangsu University ,20 (2012), pp.153-156 , in Chinese.

[16] Y. J. Sun, Y.Chen, Z. Y.Song and D.Zhou: Purification and Analysis of Cimicifugafoetida Glycoprotein. Journal of Chinese Medicinal Materials, 2(2007), pp.155-157 , in Chinese.

[17] D.J. Brasch, H.M. Chang, C.T. Chuah and L.D. Melton:. The galactan sulphate from the edible red alga Porphyra columbina. Journal of Carbohydrate Research, 97(1981), 113-125.

DOI: 10.1016/s0008-6215(00)80529-7