Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Polysaccharide Production by Tricholoma Mongolicum Imai

Xiao Tong Wu; Hai Xia Chen; Tian Xiang Han; Jing Bai; Ji Zhao; Wei Sheng Yuwen

doi:10.4028/www.scientific.net/AMR.550-553.1831

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 550-553Application of Response Surface Methodology for...

Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Polysaccharide Production by Tricholoma Mongolicum Imai

Abstract:

The fermentation medium of Tricholoma Mongolicum Imai for producing intracellular polysaccharide and extracellular polysaccharide was optimized by response surface methodology. The effects of carbon source, nitrogen source and mineral salt on polysaccharide production were examined by single factor experiments, glucose and yeast was found to be the best carbon source and nitrogen source. Based on this, the optimization of the Box-Benhnken design combined with response surface analysis showed that the optimal medium contained 40g/L glucose; 20g/L yeast extract; 0.40% KH₂PO₄; 0.40% MgSO₄.The intracellular polysaccharide production and extracellular polysaccharide in medium reached 2.0512g/L and 2.394 g/L , respectively.

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Periodical:

Advanced Materials Research (Volumes 550-553)

Pages:

1831-1836

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.550-553.1831

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Online since:

July 2012

Authors:

Xiao Tong Wu, Hai Xia Chen, Tian Xiang Han, Jing Bai, Ji Zhao, Wei Sheng Yuwen

Keywords:

Extracellular Polysaccharide, Intracellular Polysaccharide, Response Surface Methodology (RSM), Tricholoma mongolia Imai

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