Optimization of the Enzymatic Hydrolysis of Tilapia Frames

Abstract:

Tilapia frames were subjected to enzymatic hydrolysis using Flavouzryme and Papain with a ratio of 2:1. The relationship of temperature (40 to 60°C), enzyme: substrate ratio (0.5% to 4.5%), initial pH (6.0 to 8.0) and hydrolysis time (1h to 9h) to the degree of hydrolysis were determined. The enzymatic hydrolysis was optimized for maximum degree of hydrolysis using surface response methodology. The optimum conditions for enzymatic hydrolysis of tilapia frames were temperature 53°C, enzyme : substrate ratio of 3.5%, initial pH 7.2, and reaction time 7h. Under these conditions a degree of hydrolysis of 40.01% were obtained. The yield of free amino acids in the hydrolysate was 46.61mg/g tilapia frames. The flavor amino acids and essential amino acids occupied up to 31.8% and 49.0% of the total free amino acids respectively. The hydrolysate of waste tilapia frames showed good potential for applications such as protein supplementation in food system.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1387-1394
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1387
• Online since: July 2012
• Authors: He Jian Xiong, Longfei Cao, Huajun You, Qingpi Yan, Ying Ma
• Keywords: Bienzyme Hydrolysis, Degree of Hydrolysis, Response Surface Methodology (RSM), Tilapia Frames

References

[1] S. D. Zeng, J. Z. Wu, S. Y. Ou and J. Jin: Food Sci. Vol. 31 (2010), p.342

[2] Q. X. Huang, J. H. Sun, X. D. Lan, L. B. Jian, Z. F. Tong and D. K. Liao: Food Sci. Vol. 33 (2012), p.53

[3] M. Zhao and C. Y. Wu: Food Res. Dev. Vol. 28 (2007), p.48

[4] M. R. Ovissipour and M. R. Ghomi: Biotechnology in seafood production (Islamic Azad University Publication, Iran 2009).

[5] H. G. Kristinsson and B. A. Rasco: Crit. Rev. Food Sci. Vol. 40 (2000), p.43

[6] R. Šližyte, T. Rustad and I. Storrø: Process Biochem. Vol. 40 (2005), p.3680

[7] P. k. Wanasundara, R. Amarowicz, R. B. Pegg and P. J. Shand: Food Sci. Vol. 67 (2002), p.623

[8] V. M. Silva, K. J. Paek and M. D. Hubinger: J. Food Sci. Vol. 75 (2010), p. C36

[9] F. Guerard, L. Dufosse, D. Broise and A. Binet: J. Mol. Catal. B: Enzym. Vol. 11 (2001), p.1051

[10] G. Chabanon, I. Chevalot, X. Framboisier, S. Chenu and I. Marc: Process Biochem. Vol. 42 (2007), p.1419

DOI: 10.1016/j.procbio.2007.07.009

[11] T. J. Shankar, S. Sokhansan, S. Bandyopadhyay and A. S. Bawa: Food Bioprocess Technol. Vol. 3 (2010), p.498

[12] T. K. Wei and S. Manickam: Asia-Pac. J. Chem. Eng. Vol. 7 (2012), p. s125

[13] O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall: J. Biol. Chem. Vol. 193 (1951), p.265

[14] S. Nilsang, S. Lertsiri, M. Suphantharika and A. Assavanig: J. Food Eng. Vol. 70 (2005), p.571

DOI: 10.1016/j.jfoodeng.2004.10.011

[15] M. R. Ovissipour, A. M. Abedian, A. Motamedzadegan, B. Rasco, R. Safari and H. Shahiri: 18th national congress on food technology (Iran 2008)