Characterization and Selection of LAB Isolated from Inner Mongolia Traditional Meat Sausages

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A total of 69 gram-positive, catalase-negative strains isolated from Inner Mongolia traditional meat sausages were characterized in respect to their technological properties, including acid production, salt and nitrite tolerance, gas, slime and H2S production, hydrolysis of arginine and biogenic amine production. All of the isolated strains decrease the pH below 5.0 after 24h of cultivation. All isolates are able to grow with 6.5% NaCl or with 150mg L-1 NaNO2 and 51 isolates are able to grow with 10% NaCl. All isolates don’t produce gas and slime. 54 isolates don’t produce H2S. 23 isolated strains don’t produce NH3 from arginine, and 40 isolates don’t produce biogenic amine. Consolidation of these results, 8 strains show the best technological properties to be used as starter culture for sausage production. The bacterial selected are then subjected to biochemical tests using the API 50 CH and they are identified as 6 Lactobacillus planarum and 2 Lactobacillus curvatus.

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Advanced Materials Research (Volumes 554-556)

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1400-1405

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July 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] M.C. Sawitzki, A.M. Fiorentini, T.M. Bertol, Lactobacillus plantarum strains isolated from naturally fermented sausages and their technological properties for application as starter cultures, Ciencia e Tecnologia de Alimentos. 29 (2009) 340-345.

DOI: 10.1590/s0101-20612009000200016

Google Scholar

[2] M. Saeed, F.M. Anjum, T. Zahoor, Isolation and characterization of starter culture from spontaneous fermentation of sourdough, International Journal of Agriculture & Biology. 11 (2009) 329-332.

Google Scholar

[3] E. Papamanoli, N. Tzanetakis, E. Litopoulou-Tzanetaki, Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage in respect of their technological and probiotic properties, Meat Science. 65 (2003) 859-867.

DOI: 10.1016/s0309-1740(02)00292-9

Google Scholar

[4] M.G. Bonomo, A. Ricciardi, T. Zotta, Molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of Basilicata region (Southern Italy), Meat Science. 80 (2008) 1238–1248.

DOI: 10.1016/j.meatsci.2008.05.032

Google Scholar

[5] C.M.B.K. Muyanja, J.A. Narvhus, J. Treimo, Isolation, characterisation and identification of lactic acid bacteria from bushera: a Ugandan traditional fermented beverage, International Journal of Food Microbiology. 80 (2003) 201–210.

DOI: 10.1016/s0168-1605(02)00148-4

Google Scholar

[6] M. Hugas, M. Garriga, T. Aymerich, Biochemical characterization of lactobacilli from dry fermented sausages, International Journal of Food Microbiology. 18 (1993) 107-113.

DOI: 10.1016/0168-1605(93)90215-3

Google Scholar

[7] M. Kostinek, I. Specht, V.A. Edward, Characterisation and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures, International Journal of Food Microbiology. 114 (2007) 342–351.

DOI: 10.1016/j.ijfoodmicro.2006.09.029

Google Scholar

[8] H. Albano, C.A. van Reenen, S.D. Todorov, Phenotypic and genetic heterogeneity of lactic acid bacteria isolated from 'Alheira, a traditional fermented sausage produced in Portugal. Meat Science, 2009 (82): 389–398.

DOI: 10.1016/j.meatsci.2009.02.009

Google Scholar

[9] S. Ammor, C. Rachmanb, S. Chaillouc, Phenotypic and genotypic identification of lactic acid bacteria isolated from a small-scale facility producing traditional dry sausages, Food Microbiology. 22 (2005) 373–382.

DOI: 10.1016/j.fm.2004.11.005

Google Scholar

[10] R. Coppola, B. Giagnacovo, M. Iorizzo, Characterization of lactobacilli involved in the ripening of soppressata molisana, a typical southern Italy fermented sausage, Food Microbiology. 15 (1998) 347–353.

DOI: 10.1006/fmic.1997.0179

Google Scholar

[11] U. Schillinger, F.K. Lucke, Identification of lactobacilli from meat and meat products, Food Microbiology. 4(1987) 199-208.

DOI: 10.1016/0740-0020(87)90002-5

Google Scholar

[12] S. Bover-Cid, W.H. Holzapfel, Improved screening procedure for biogenic amine production by lactic acid bacteria, International Journal of Food Microbiology. 53 (1999) 33–41.

DOI: 10.1016/s0168-1605(99)00152-x

Google Scholar

[13] M. Hugas, J. M. Monfort, Bacterial starter cultures for meat fermentation, Food Chemistry. 59 (1997) 547–554.

DOI: 10.1016/s0308-8146(97)00005-8

Google Scholar

[14] M.S. Ammor, B. Mayo, Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production: an update, Meat Science. 76 (2007) 138-146.

DOI: 10.1016/j.meatsci.2006.10.022

Google Scholar

[15] A. Rovira, J.C. Nieto, A. Rodríguez, Characterization and selection of lactobacilli isolated from Spanish fermented sausages, Microbiologia. 13 (1997) 201-208.

Google Scholar

[16] H. J. Buckenhüskes, Selection criteria for lactic acid bacteria to be used as starter cultures for various food commodities, FEMS Microbiology Reviews. 12 (1993) 253–271.

DOI: 10.1016/0168-6445(93)90067-j

Google Scholar

[17] P.M. Makela, Fermented sausage as a contamination source of ropy slime-producing lactic acid bacteria. J. Food Protect. 55 (1992) 48-51.

DOI: 10.4315/0362-028x-55.1.48

Google Scholar

[18] I. Erol, G. Hilbedrandt, J. Wiegner, The Isolation of Lactobacillus curvatus as a cause of H2S production from smoked and vacuum packaged fermented sausage, GIDA. 19 (1994) 271-275.

Google Scholar

[19] H.M.L.J. Joosten, M.D. Northolt, Detection, growth, and amine-producing capacity of Lactobacilli in cheese, Applied and Environmental Microbiology. 9 (1989) 2356-2359.

DOI: 10.1128/aem.55.9.2356-2359.1989

Google Scholar

[20] G. Suzzi, F. Gardini, Biogenic amines in dry fermented sausages: a review, International Journal of Food Microbiology. 88 (2003) 41– 54.

DOI: 10.1016/s0168-1605(03)00080-1

Google Scholar

[21] K. Rantsiou, E.H. Drosinos, M. Gialitaki, Use of molecular tools to characterize Lactobacillus spp. Isolated from Greek traditional fermented sausages, International Journal of Food Microbiology. 112 (2006) 215–222.

DOI: 10.1016/j.ijfoodmicro.2006.09.001

Google Scholar

[22] M.J. Benito , M.J. Serradilla, S. Ruiz-Moyano, Rapid differentiation of lactic acid bacteria from autochthonous fermentation of Iberian dry- fermented sausages, Meat Science. 80 (2008) 656–661.

DOI: 10.1016/j.meatsci.2008.03.002

Google Scholar

[23] K. Rantsioua, E.H. Drosinos, M. Gialitaki, Molecular characterization of Lactobacillus species isolated from naturally fermented sausages produced in Greece, Hungary and Italy, Food Microbiology. 22 (2005) 19–28.

DOI: 10.1016/j.fm.2004.05.001

Google Scholar