Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell In Vitro by E-Cadherin-Fc as a New Smart Biological Materials

Nan Jiang; Jian Bin Xu; Jun Yang

doi:10.4028/www.scientific.net/AMR.554-556.1785

Paper Titles

Functional Role of HIF-1α in Hypoxic Preconditioning-Induced Neuroprotection against Focal Cerebral Ischemia
p.1762

Effect of Fibrinolytic Compound GDG on Crystal Structure of Lysozyme
p.1768

Study on the Synthesis and Resolution of Dl-Ephedrine
p.1776

Simultaneous Determination of Sulfadiazine and Sulfamethoxazole in Synthetic Samples and Pharmaceutical Tablets Using HPLC-DAD Combined with Second-Order Calibration Method
p.1781

Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell In Vitro by E-Cadherin-Fc as a New Smart Biological Materials
p.1785

The Study of Interference with Ruyanneixiao Cream on Hemorheology and Mammary Microcirculation of Mammary Precancer Rats
p.1789

Anti-Fibrotic Roles of Oviductus Ranae in Rats with Hepatic Fibrosis
p.1794

Problems and Countermeasures of Experimental Animals for Medicinal Use
p.1799

The Surface Acid Properties of TS-1
p.1803

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Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell In Vitro by E-Cadherin-Fc as a New Smart Biological Materials

Abstract:

There are many research shows that the umbilical cord was a “store house” of stem cells. Human umbilical cord mesenchymal stem cell(HUMSC) was considered to be an idea seed cell for cell therapy and cell models construction used in drug development. But the regulation of HUMSC proliferation and differentiation in vitro is facing challenges. This research aimed to construct a biomimic microenvironment using bioactive materials including the fusion protein of E-cadherin-Fc and promote HUMSC proliferation. The adhersion and proliferation of HUMSC measured by MTT assay, were obviously enhanced on E-cadherin-Fc coated surfaces, comparing with these on the gelatin coated surfaces and conventional conditions. Meanwhile, the expression of CD105 assayed by flow cytometry, showing that the pluripotency of HUMSC cultured on the E-cadherin-Fc coated surfaces were higher than that in the tissue treated plate. From the above, the establishment of E-cadherin-Fc microenvionment is beneficial for HUMSC proliferation in vitro.