Chiral Separation of Non-Natural Amide Amino Acid by Capillary Electrophoresis with CD Derivations as Chiral Selective Material

Abstract:

A capillary electrophoresis (CE) method for the separation of four kinds of enantiomers of non-natural carboxylic amino acid was built while hydroxypropyl-β- cyclodextrin (HP-β-CD) derivations as chiral selective material. Several different β-CD derivatives were tested for the chiral separation of non-natural carboxylic amino acid, and it was proved that HP-β-CD could show better chiral selectivity. The separation of enantiomers of amino acid was obtained by CE in a 50-μm i.d.×60 cm (effective length 45 cm) fused-silica capillary at 18 kV voltage, while 10 mM phosphate acted as running buffer and HP-β-CD served as selective material. The detective wavelength was set at 254 nm.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 824-827
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.824
• Online since: July 2012
• Authors: Bao Hui Li
• Keywords: Amide Amino Acid., Capillary Electrophoresis, Chiral Selective Material, Isomer Separation, Non-Natural

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