Study on Culture and Mutational Screening of Pholiota Adipose

Abstract:

Appropriate seeding medium was chosen in the paper : potatoes 50g , wood chips25g, glucose10g, peptone5g, KH2PO41.0g, MgSO4 0.5g, Vitamin B1 a little, 500ml water. wood chips 80％, Bran 17％, Gypsum powder 2％, Sucrose 1％, Feed-water ratio 1：1. Pholiota adipose was cultured at 15°C-18°C, relative moisture 60%-80% for 15-25days. The spore of Pholiota adipose was mutagenesis with Nitrite，5minutes，Nitrite 0.1mol/L，Strong ability to obtain mycelium of Pholiota adipose. The strain mutagenesis by 0.05mol / L sodium nitrite could grow mutagenicity colony, When sodium nitrite concentration was higher than 0.1mol / L, the mycelium of Pholiota adipose began to dissolve.

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 337-340
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.337
• Online since: December 2012
• Authors: Sheng Quan Xie, Yan Ling Quan, Yi Sheng Liu, Chun Guang Zhang
• Keywords: Chemical Mutagenesis, Fungi, Pholiota Adipose, Strain Breeding

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