Iron Salt Concentration Effect to the Precipitation of Iron Oxide Nanoparticles Conjugated Antibody

Mohamad Nor Noorhashimah; Abdul Razak Khairunisak; Azlan Abdul Aziz; Rahmah Noordin

doi:10.4028/www.scientific.net/AMR.620.268

Paper Titles

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Microstructure Evolution of Sn-3.5Ag-1.0Cu-0.5Ni/Cu System Lead Free Solder under Long Term Thermal Aging
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Iron Salt Concentration Effect to the Precipitation of Iron Oxide Nanoparticles Conjugated Antibody
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Morphology and Intermetallics Study of (Sn-8Zn-3Bi)-1Ag Solder under Liquid-State Aging
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 620Iron Salt Concentration Effect to the...

Iron Salt Concentration Effect to the Precipitation of Iron Oxide Nanoparticles Conjugated Antibody

Abstract:

This study covers the effect of ferrous chloride (FeCl₂) concentration on the formation of iron oxide nanoparticles (IONPs) via the precipitation method. IONPs with appropriate surface functionalization were synthesized in order to obtain a stable colloidal IONPs (ferrofluid) for the conjugation process. The electrostatic repulsion using percloric acid (HClO₄) and steric stabilization using silane polyethelene glycol (SiPEG) were generated. The optimum concentration of FeCl₂ was at 0.3 M. From the transmission electron microscopy (TEM) image, the size of IONPs obtained was ~13 nm. The stable IONPs were then conjugated to the antibody and tested in the lateral flow immunoassay as the labelling agent. The conjugated IONPS with the antibody was proven sensitive to the Brugian Filiarisis disease.

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Periodical:

Advanced Materials Research (Volume 620)

Pages:

268-272

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.620.268

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Online since:

December 2012

Authors:

Mohamad Nor Noorhashimah, Abdul Razak Khairunisak, Azlan Abdul Aziz, Rahmah Noordin

Keywords:

Iron Oxide Conjugated Antibody, Lateral Flow Immunoassay, Ron Oxide Nanoparticles

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