Preparation, Characterization and Phototoxicity of Photosan-Loaded Hollow Silica Nanospheres for Photodynamic Therapy

Abstract:

In this study, Photosan (PS) was loaded onto hollow silica nanospheres using one-step wet chemical-based synthetic route method. Polydispersive index of the Photosan-loaded hollow Silica Nanospheres (PS-loaded NSs) is 0.121±0.01. The photobiological activity of the PS-loaded NSs was evaluated on human cholangiocarcinoma QBC939 cells. Cells were incubated with free PS or PS-loaded NSs for 2 h and then washed with phosphate-buffered saline. Culture medium was added to the wells containing the cells. Finally, the cells were exposed to red light (630 nm) with a light dose of 10J/cm². The cellular viability was determined after 24 h of incubation. PS-loaded NSs and free PS eliminated about 96.4%±2.0% and 54.7%±1.9% of QBC939 cells, respectively. The phototoxicity was time dependent up to 2 h and concentration dependent at 1-7.5 mg/l. The cells viability decreased with the increase of the light dose in the range of 5-15 J/cm². In conclusion, PS-loaded NSs are the release systems that promise photodynamic therapy use.

• Periodical: Advanced Materials Research (Volumes 622-623)
• Pages: 930-938
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.622-623.930
• Online since: December 2012
• Authors: Xiao Feng Deng, Yu Wen, Liang Wu Lin, Li Xiong, Xiong Ying Miao
• Keywords: Cholangiocarcinoma, Nanospheres, Photodynamic Therapy, Photosensitizer, Phototoxicity

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