Spectroscopic Studies on the Binding Properties of Ofloxacin and Human Telomeric G-Quadruplex DNA

Abstract:

The binding of ofloxacin with human telomeric G-quadruplex DNA, Htel-G4-DNA and Htel-3-G4-DNA were examined by Fluorescence and CD spectroscopic methods. In the Fluorescence emission spectral analysis, the addition of ofloxacin induced significant quenching on the fluorescence emission of TO-G4-DNA complex. The fluorescence spectral analysis indicated that ofloxacin exhibited higher binding affinity and binding intensity to Htel-G4-DNA than Htel-3-G4-DNA. In the CD spectral analysis, the interaction with ofloxacin did not disturb the characteristic absorption of Htel-G4-DNA at 290 nm corresponding to its antiparallel form, and only slightly increased the positive absorption at 270 nm as shoulder peak, which suggests the antiparallel structure of G-quadruplex can remain stable in the presence of ofloxacin

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1062-1065
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1062
• Online since: January 2013
• Authors: Xu Jian Luo, Qi Pin Qin, Yu Lan Li, Yan Yang
• Keywords: FID Assay, G-Quadruplex DNA, Ofloxacin, Spectroscopy

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