Study of a upp-Based Counterselective Method for Large-Scale Deletion of Genome Fragments in Bacillus subtilis

Xue Yu Zhang; Jing Fu; Tao Chen

doi:10.4028/www.scientific.net/AMR.634-638.1076

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 634-638Study of a upp-Based Counterselective Method for...

Study of a upp-Based Counterselective Method for Large-Scale Deletion of Genome Fragments in Bacillus subtilis

Abstract:

The generation of an engineering strain with reduced genome relies on efficient methods for marker-free gene knockout. In this study, research on the efficiency of deletion of genome fragments with upp-based counterselective method in Bacillus subtilis was carried out. Using this method, the 3.053 Kb, 12.414 Kb and 34.148 Kb fragments of the skin element were marker-free deleted successfully, with the efficiency of 27%, 11.4% and 25%, respectively. Here we demonstrate that this method is an efﬁcient genetic tool for large genome fragments marker-free deletion.

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Periodical:

Advanced Materials Research (Volumes 634-638)

Pages:

1076-1080

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.634-638.1076

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Online since:

January 2013

Authors:

Xue Yu Zhang, Jing Fu, Tao Chen

Keywords:

Bacillus subtilis, Large Genome Fragments Knockout, Marker-Free Manipulation

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