Optimization of Culture Medium and Conditions for Bacillus coagulans T242 Producing Thermostable Lactase

Abstract:

A thermotolerant lactase-producing strain Bacillus coagulans T242 was obtained in our previous work, its media and culture conditions were investigated and optimized for enhanced production of lactase in the present work. Results showed medium containing 1.5% lactose, 1.0% peptone, 1.5% yeast extract, 0.7% MgSO4, and pH 8.0, was the optimum medium; And its optimum culture conditions were the age of bacterial culture 36 h, inoculation volume 2%, liquid medium volume loaded 30 mL/250 mL flask, 60 °C, 36 h. When cultured at the optimum condition Bacillus coagulans T242 yielded the maximum of 1.21U/mL lactase activity.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1259-1262
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1259
• Online since: January 2013
• Authors: Fang Qian, Shu Juan Jiang, Tao Liu, Guang Qing Mu
• Keywords: Bacillus coagulans, Culture Conditions, Culture Medium, Lactase

References

[1] Q. Husain: Crit. Rev. Biotechnol. Vol. 30 (2010), p.41–62.

[2] A.R. Park, D.K. Oh: Appl. Microbiol. Biotechnol. Vol. 85 (2010), p.1279–86.

[3] P.S. Panesar, R. Panesar, R.S. Singh, J.F. Kennedy and H.J. Kumar: Chem. Technol. Biotechnol. Vol. 81 (2006), p.530–43.

[4] F. Qian, S.J. Jiang, T. Liu and G.Q. Mu: submitted to Advanced Materials Research (2012).