Extraction, Purification and Characterization of Polysaccharides from Clam (Ruditapes philippinarum)

Abstract:

Polysaccharides were extracted from clam (Ruditapes philippinarum) (RPPS) by enzymatic method, and the crude polysaccharides were purified by filtration DEAE-Sepharose Fast Flow chromatography and Sephacryl S-400 size-exclusion chromatography. Two main fractions of RPPS-1a and RPPS-1b were separated. Furthermore, characterizations, such as polysaccharides composition and thermal properties, were analyzed. The results showed that polysaccharides extraction rate was 0.89%, and polysaccharides content was 62.5%. The polysaccharides were comprised of aminosu-gars, uronic acid, fucose and sulfate, which accounted for 0.27%, 59.30%, 0.75% and 1.33%, respectively. According to the infrared spectra, both RPPS-1a and RPPS-1b were polysaccharides containing -CHO and-COOH, and RPPS-1a had an additional sulfate groups. DSC analysis revealed that RPPS-1a might have straight-chain crystals, and RPPS-1b might contain more branch-chain crystals.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1236-1240
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1236
• Online since: January 2013
• Authors: Yi Liu, Qian Cheng Zhao, Zhi Bo Li, Hong Ye Wang
• Keywords: Characterization, CLAM, Extraction, Polysaccharide, Purification, Ruditapes philippinarum

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