Bacterial Diversity and Community Structure Analysis for Two Samples of Mine Drainage from Shimen Realgar Mine, China

Abstract:

Two samples of mine drainage, named SY and XY respectively, were collected from Shimen Realgar Mine, Hunan province, China. In addition to physic-chemical analysis, bacterial diversities and community structures of these samples were described at genetic level by restricted fragment length polymorphisms (RFLP). A total of 66 and 76 different operational taxonomic units (OTUs) patterns were obtained from 160 clones respectively. Total 30 patterns, which were dominant or both appeared in these two samples, were re-amplified and sequenced. The sequence data and phylogenetic analysis showed that uncultured bacteria represented 62% of the population in samples due to the high concentration of arsenic. And almost all of the affiliated sequences have very close relationships with extreme environment. However, samples SY and XY contained more diverse colonies of bacteria. These diversities were characterized by the reciprocal of Simpson’s index (1/D).

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 246-252
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.246
• Online since: January 2013
• Authors: Yu Yang, Li Yang, Xiao Ming Xiang
• Keywords: Arsenic, Bacterial Diversity, RFLP, Shimen Realgar Mine

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