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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Analysis of Synonymous Codon Usage Bias in D15...

Analysis of Synonymous Codon Usage Bias in D15 Gene Encoded Surface Antigen of Riemerella Anatipestifer

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Abstract:

In order to provide a basis for understanding the evolutionary relationship and pathogenesis of Riemerella anatipestifer and selecting a appropriate host expression systems to improve the expression of target gene in vivo and in vitro, we identified the codon bias in the newly confirmed D15 gene of Riemerella anatipestifer ATCC 11845 strain and performed comparative analysis of the codon usage bias between D15 gene in R. anatipestifer and the other 10 referenced Flavobacteriaceaes by a series of online bioinformatics softwares. The results revealed that the synonymous codons with A and T at the third codon position had widely usage in the codon of D15 gene of R. anatipestifer. In addition, there were 70 rare codons in the ORF of the D15 of R. anatipestifer, and 32 codons showing distinct usage differences between R. anatipestifer and E. coli, 30 codons between R. anatipestifer and Homo sapiens, 16 codons between R. anatipestifer and yeast, indicated the yeast expression system may be more suitable for the expression of R. anatipestifer genes. The extent of codon usage bias in the D15 gene in R. anatipestifer was highly correlated with the gene expression level, therefore the results may provide useful information for gene classiﬁcation and functional studies.

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Biotechnology, Chemical and Materials Engineering II

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

597-605

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.597

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Online since:

January 2013

Authors:

Bin Feng, De Kang Zhu, Xiao Jia Wang, An Chun Cheng, Ming Shu Wang

Keywords:

Codon Usage Bias, D15 Gene, Riemerella anatipestifer

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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