Detection of L-Tert-Leucine in the Enzyme-Catalyzed Reaction System

Sheng Deng; Li Cui; Li Min Ma

doi:10.4028/www.scientific.net/AMR.641-642.717

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Detection of L-Tert-Leucine in the...

Detection of L-Tert-Leucine in the Enzyme-Catalyzed Reaction System

Abstract:

Direct Determination of L-Ter-Leucine in Enzyme Catalytic Reaction System by HPLC Was Studied. the Detection Were Performed on a Kromasil 700-5C18 Column Using a Eluant Containing 0.25% (NH4)H2PO4 and 100% Methanol (V((NH4)H2PO4):V(methanol)=100:5) with the Flow Rate of 0.8 Ml/min at,detection Wavelength of 205nm. there Was a Good Line Correlation between Peak Area and Contents in the Rang of 0.2-10 Mg/ml, the Correlation Coefficient Was 0.9986, the Average Recovery Was 98.88% with a Relative Stand Deviation of 0.78% (n=5). this Method Is Simple, Stable, Accurate and Reliable for the Quality Control of L–ter-Leucine.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

717-720

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.717

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Online since:

January 2013

Authors:

Sheng Deng, Li Cui, Li Min Ma

Keywords:

Enzyme Catalytic, High Performance Liquid Chromatography (HPLC), L-Ter-Leucine

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