D-Lactic Acid Production from Xylose in Engineered Escherichia coli SZ470

Abstract:

WD100, knocked out adhE of Escherichia coli SZ470 and inserted ldhA into Escherichia coli WD01, was genetically engineered to utilize xylose. D-lactate production was investigated for shake flask cultures with xylose. In 64h WD100 produce 10.1g/L D-lactate in the shaking flask And it consumed 25g/L xylose during the ending of fermentation.This volumetric productivity with xylose is 0.14 g·L^-1·h^-1.Because of pyruvate decarboxylase (poxB) expressed in flask fermention,acetate production was up to 4.7g/L.Succinate,formate,ethanol was also produced as a minor product during fermentation.

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 721-724
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.721
• Online since: January 2013
• Authors: Zhao Min Zheng, Tian Tian, Jin Hua Wang, Yong Ze Wang, Sheng De Zhou
• Keywords: Escherichia coli, Lactic Acid Fermentation, Poly(lactic acid) (PLA), Xylose

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