Effect of Tumstatin T-7 Peptide on HepG-2 Cells and Human Umbilical Vein Endothelial Cells

Abstract:

The direct anti-tumor T-7 peptide of tumstatin was obtained to study its effect on human hepatoma cells (HepG-2) and human umbilical vein endothelial cells (ECV304). T-7 peptide was synthesized and its purity was up to 98.5%. MTT assay, growth curve, transmission electron microscopy(TEM) were used to detect the proliferation inhibition and pro-apoptotic function. MTT experiments and growth curve experiments showed that the survival of human hepatoma cells (HepG-2) decreased in a time- and dose-dependent way with the concentration of T-7 peptide increased. T-7 peptide can induce apoptosis of HepG-2 significantly. Apoptosis features were observed by TEM. However, the inhibition of T-7 peptide on human umbilical vein endothelial cells (ECV304) was weaker. Experiments showed thatT-7 peptide can affect HepG-2 cells. It had a certain therapeutic effect on human hepatocellular carcinoma. T-7 peptide have little effect on ECV304 cells, which means it didn’t influence the formation of tumor angiogenesis and normal cells

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 744-747
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.744
• Online since: January 2013
• Authors: Shu Jing Wang, Fei Wang, Jia Liu, Shan Jiang, Ning Chen, Lin Liu, Ying Zhao, Xiao Dan Zhang
• Keywords: Angiogenesis, Cell Apoptosis, Cell Proliferation, Hepatocarcinoma, Tumstatin

References

[1] Hamano Y, Zeisberg M, Sugimoto H, et al. Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaV beta 3 integrin[J]. CancerCell, 2003, 23(6): 589-601.

DOI: 10.3410/f.1014883.195864

[2] Saus J, Wieslander J, Langeveld J P, Quinones S. Identification of the goodpasture antigen as the alpha 3(IV) chain of collagen IV[J]. J Biol Chem, 1988, 263(26): 13374-13380.

DOI: 10.1016/s0021-9258(18)37714-7

[3] G Collo, MS Pepper. Endothelial cell integrin alpha 5 beta 1 expression is modulated by cytokines and during migration in vitro[J]. J Cell Sci, 1999, 112(5): 569-578.

DOI: 10.1242/jcs.112.4.569

[4] Floquet N, Pasco S, Ramont L, et al. The antitumor properties of the alpha3(IV)-(185-203) peptide from the NC1 domain of type IV collagen (tumstatin) are conformation-dependent[J]. J Biol Chem, 2004, 279(3): 2091-2100.

DOI: 10.1074/jbc.m307736200

[5] Pedraza-Farifia LG. Mechanisms of oncogenic cooperation in cancer initiation and metastasis[J]. Yale J Biol Med, 2006, 79(3-4): 95-103.