Enzymatic Transformation from Protopanaxadiol Ginsenoside Rb1 into Rare Ginsenoside C-K and its Anti-Cancer Activity

Li Min Liao; Yang Zhang; Shu Fang Lin; Su Bin Hong; Yi Lin

doi:10.4028/www.scientific.net/AMR.641-642.752

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Enzymatic Transformation from Protopanaxadiol...

Enzymatic Transformation from Protopanaxadiol Ginsenoside Rb1 into Rare Ginsenoside C-K and its Anti-Cancer Activity

Abstract:

Rare ginsenoside C-K was prepared using enzymatic transformation with snailase from main ginsenoside Rb1 at the following conditions: material ratio 62.14:10, pH 4.0, incubation at 46.40 °C for 15 h. After chromatography by silica gel column and crystal formation, C-K with more than 95% purity was obtained from the production of enzymatic transformation. The yield of C-K reached 63.02% after optimization, representing an about 9% increase than before. In addition, C-K we prepared exhibited an obvious inhibitory effect on the proliferation of human lung adenocarcinoma cancer cell A549 and human hepatoma cell HepG-2.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

752-755

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.752

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Online since:

January 2013

Authors:

Li Min Liao, Yang Zhang, Shu Fang Lin, Su Bin Hong, Yi Lin

Keywords:

Anticancer Activity, Enzymatic Transformation, Rare Ginsenoside C-K, Rb1, Snailase

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References

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