Heterologous Expression of Production of T10, C12-CLA Linoleic Acid Isomerase Gene from Propionibacterium acnes

Abstract:

The linoleic acid isomerase enzyme from Propionibacterium acnes, which catalyzed the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating trans-10, cis-12 conjugated linoleic acid (t10, c12 CLA) was cloned and overexpressed in Escherichia coli using expression system of pCold-SUMO. The gene was expressed under T7 promotor with a fusion partner of 6×His. Tag at its 5'terminal. After induction by 0.2mM IPTG for 18 h, the recombinant enzyme are expressing in the cytoplasm. The 1275 bp gene from P. acnes encode a linoleic acid isomerase protein with 424 amino acids (55KD).

• Periodical: Advanced Materials Research (Volumes 641-642)
• Pages: 765-768
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.641-642.765
• Online since: January 2013
• Authors: Xue Luo, Lan Wei Zhang, Hong Bo Li, Shu Mei Wang, Chao Hui Xue
• Keywords: Biotransformation, Linoleic Acid Isomerase Gene, Recombinant Plasmid

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