Sample Preparation for HPLC Determination of Free and Oligosaccharides-Bound Sialic Acid in Bovine Colostrum

Min Peng Zhu; Su Hong Li; Xiu Hong Zhao; Peng Guan

doi:10.4028/www.scientific.net/AMR.641-642.882

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Sample Preparation for HPLC Determination of Free...

Sample Preparation for HPLC Determination of Free and Oligosaccharides-Bound Sialic Acid in Bovine Colostrum

Abstract:

An analytical method was developed for determining free and oligosaccharide-bound sialic acids in bovine colostrum by HPLC. The condition of acid hydrolysis for sample and sialic acids derivatization were investigated. The sialic acids were released from their parent oligosaccharides by acid hydrolysis using 40mL 0.1mol/L sulphuric acid for 100min. They were then derivatized using O-phenylenediamine (OPD) at 70°C for 40 min, the determination of the derivative could be carried out by UV detection. The total content of free and oligosaccharide bound sialic acids in bovine colostrum milk were 941.95μg/mL. The developed method is validated on various dairy products such as bovine milk and milk-based products.

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Periodical:

Advanced Materials Research (Volumes 641-642)

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882-885

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.882

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Online since:

January 2013

Authors:

Min Peng Zhu, Su Hong Li, Xiu Hong Zhao, Peng Guan

Keywords:

Bovine Colostrum, High Performance Liquid Chromatography (HPLC), Hydrolysis, Salic Acid

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