The Exploitation and Simulation of a Microfluidic Device Research on the Response of Cell Fluid Shear Stress

Yuan Chen Wei; Bao Dong Bai

doi:10.4028/www.scientific.net/AMR.647.357

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The Exploitation and Simulation of a Microfluidic Device Research on the Response of Cell Fluid Shear Stress
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The Evaluation of Process of Bioremediation of Oil-Polluted Soils by Different Strains of Pseudomonas
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 647The Exploitation and Simulation of a Microfluidic...

The Exploitation and Simulation of a Microfluidic Device Research on the Response of Cell Fluid Shear Stress

Abstract:

This paper provides an economical and high efficient kind of microfluidic chip device which can investigate cells' response under different magnitude of fluid shear stress (FSS) at the same time. The experimental results of a large number of test groups in the same environmental conditions are more persuasive for conclusion. Cells are simultaneously cultured in different width of micro channel, and we verified the cell's shear force with a COMSOL simulation. In the experiment, Human vascular endothelial cell which possess apparent FSS response is selected to verify the reliability of the device. The results of the experiment show that cell response to FSS has obvious morphological differences in different test group.

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Periodical:

Advanced Materials Research (Volume 647)

Pages:

357-362

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.647.357

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Online since:

January 2013

Authors:

Yuan Chen Wei, Bao Dong Bai

Keywords:

COMSOL Computational Fluid Dynamics (CFD), Fluid Shear Stress (FSS) Stimulation, Microfluidics Chip, Vascular Endothelial Cell (VEC)

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References

[1] Pittelkow MR. New techniques for the in vitro culture of human skin keratinocytes and perspectives on heir use for grafting of patients with extensive burns [J] . Nayo Clin Proc, 1986, 61: 771.

DOI: 10.1016/s0025-6196(12)64815-0

Google Scholar

[2] Dewey CF, Jr. Effects of fluid flow on living vascular cells [J] . ASM EJ Bio Mech Eng, 1984, 106: 31.

Google Scholar

[3] Ives CL, Ekin SG, Mcintire LV. Mechanical effects on endothelial cell morphology. In vitro assessment. In vitro cellular and developmental [J]. Biology, 1986, 22: 500.

DOI: 10.1007/bf02621134

Google Scholar

[4] Sholley MM, Gimbrone MA Jr, Controm RS. Cellular migration and replication in endothelial regeneration: study using madiated endot helial vulture [J]. Lab Invest, 1997, 36: 18.

Google Scholar

[5] Ando J, Kamiya A. Blood flow and vascular endot helial cell function [J]. Pront Med Biol Eng, 1993, 5: 245.

Google Scholar

[6] Moeller, H.; Mian, M. K.; Shrivastava, S.; Chung, B. G.; Khademhosseini, A. A microwell array system for stem cell culture. Biomaterials. , 2008, 29, (6), 752-763.

DOI: 10.1016/j.biomaterials.2007.10.030

Google Scholar

[7] J. El-Ali, P.K. Sorger and K.F. Jensen, Nature, 2006, 442, 403–411.

Google Scholar

[8] J.B. Shao, L. Wu, J. Z. Wu, Y. H. Zheng, H. Zhao, Q. H. Jin and J. L. Zhao, Lab Chip, 2009, 9, 3118–3125.

Google Scholar

[9] Information on http: /comsol. cntech. com. cn.

Google Scholar