A New Fluorescence Method for the Determination of Hsa Using Graphite Oxide as Nanoprobe

Abstract:

Graphite oxide (GO) was prepared by Hummer procedure, and can be dispersed to obtain stable GO nanocolloid solution by ultrasonic wave. The GO exhibited a weak fluorescence peak at 425 nm in pH 4.6 HAc-NaAc buffer solution. Upon addition of human serum albumin (HSA), it combined with GO nanoprobe to form big HSA-GO particles that caused the fluorescence peak increasing. The increased fluorescence intensity was linear to HSA concentration in the range of 2-200 μg/mL. Thus, a new and simple fluorescence method was proposed for the determination of HSA in real sample.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 769-773
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.769
• Online since: January 2013
• Authors: Xin Hui Zhang, Li Li Xu, Ai Hui Liang, Zhi Liang Jiang
• Keywords: Fluorescence Method, Graphite Oxide, HSA

References

[1] Y. G. Yang, C. M. Chen, Y. F. Wen, Q. H. Yang, and M. Z. Wang. New Carbon Mater. Vol. 23 (2008), p.193.

[2] T. Szabo, E. Tombacz, and E. Lles. Carbon Vol. 44 (2006), p.537.

[3] A. Lerf, A. Buchsteiner, and J. Pieper. J. Phy. Chem. Solids Vol. 67 (2006), p.1106.

[4] T. Nakajima, A. Mabuchi, and R. Hagiwara. Carbon Vol. 26 (1988), p.357.

[5] H. He, J. Klinowski, and M. Forster. Chem. Phy. Lett. Vol. 287 (1998), p.53.

[6] Y. W. Zhu, S. Murali, W. W. Cai, X. S. Li, J. W. Suk, J. R. Potts, and R. S. Ruoff. Adv. Mater. Vol. 22 (2010), p.3906.

[7] Y. J. Song, K. G. Qu, C. Zhao, J. S. Ren, and X. G. Qu. Adv. Mater. Vol. 22 (2010), p.2206.

[8] T. Kuila, S. Bose, P. Khanra, A. K. Mishra, N. H. Kim, and J. H. Lee. Biosen. Bioelectron. Vol. 26 (2011), p.4637.

[9] C. Z. Zhu, S. J. Guo, Y. X. Fang, and S. J. Dong. ACS Nano Vol. 4 (2010) p.2429.