Extraction of Lipase from Rice Bran Using Aqueous Two-Phase System

Qing Yun Wang; Qin Lu Lin; Xiao Hao Li

doi:10.4028/www.scientific.net/AMR.651.384

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 651Extraction of Lipase from Rice Bran Using Aqueous...

Extraction of Lipase from Rice Bran Using Aqueous Two-Phase System

Abstract:

Aqueous two-phase system (ATPS) is an efficient and economical bioseparation technique. In this paper, the PEG2000/(NH₄)₂SO₄ aqueous two-phase system was used to extract lipase from rice bran, and the best conditions were 24% PEG2000, 14% (NH₄)₂SO₄, 15% addition of crude enzyme and pH 7.5. At this condition, the partition coefficient and activity recovery were highest and the activity recovery could achieve 90.29%.

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Periodical:

Advanced Materials Research (Volume 651)

Pages:

384-388

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.651.384

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Online since:

January 2013

Authors:

Qing Yun Wang, Qin Lu Lin, Xiao Hao Li

Keywords:

Aqueous Two-Phase, Extraction, Lipase, PEG/(NH₄)₂SO₄, Rice Bran

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References

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