Isolation of a Novel Chiral Phytoestrogen Breviflavone B from Epimedium Herb by a New Approach of Liquid Chromatography

Abstract:

Breviflavone B is a novel chiral phytoestrogen that mimics the biological functions of 17β-estradiol (E2), an estrogen which is naturally produced in our body. It has exhibited a much higher estrogen receptor (ER) bioactivity than E2 or other phytoestrogens. Breviflavone B specifically activates ER at a low dose without activating other steroid receptors, and inhibits estrogen-stimulated breast cancer cell proliferation at high doses, making it a potential treatment option for breast cancer patients. In this report, a new liquid chromatography aprroach has been developed to isolate Breviflavone B from Epimedium Brevicornum herbal extracts. After passing through DIAION HP20 resin, the ethanolic extract of Epimedium Brevicornum was firstly fractionalized by a Diol column, then further separated by a C18 column using preparative HPLC, and finally purified by an ODS-MCR-HPS column in HPLC under gradient mode. In each stage of separation, the ER bioactivities of the each fraction were measured by estrogen receptor-driven reporter gene bioassays to guide the chromatographic fractionations. The results showed that the new combination of Diol and C18 and ODS-MCR-HPS column chromatography provided better separation selectivity than our previously-reported C18 column chromatography for isolation of Breviflavon B from Epemidium herbs. Pure Breviflavon B was successfully isolated from the ethanolic extract of Epimedium Brevicornum herb via this new approach of chromatographic fractionations guided by the bioassays.

• Periodical: Advanced Materials Research (Volume 663)
• Pages: 303-307
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.663.303
• Online since: February 2013
• Authors: Shu Fang Soh, Jia Yin Tay, Jun Li, Lian Qian, Xiao Xing Yin, Qun Li Wei, Hai Bin Yang, Li Sha Wang, De Ming Wang, E.L. Yong, Yin Han Gong
• Keywords: Liquid Chromatography, Nature Product Separation, ODS-MCR-HPS, TCM

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