Effect of Deicing Salt on Catalase Activity and Malondialdehyde Contents in Gills and Muscle Tissues of Zebrafish (Danio rerio)

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The present study was designed to elucidate the Catalase (CAT) activity and malondialdehyde (MDA) contents in gills and muscle tissues of Zebrafish in exposure to different concentrations of deicing salt. According to the value of 96 h LC50, five different concentration treatments (4.30, 5.38, 6.73, 8.41 and 10.51 g/L) of deicing salt were set up with no application of deicing salt (0 g/L) as control treatment, and the activity of CAT and MDA in the gills and muscle tissues of Zebrafish were measured at 1d, 4d, and 7 d exposure. The results showed that the 96 h LC50 value of deicing salt for Zebrafish was 13.49 g/L and the safe concentration was 4.26 g/L. The CAT activity in muscle tissues of Zebrafish was activated significantly (P<0.01) at 1d, and was inhibited significantly with the concentration of 10.51 g/L (P<0.05) by comparing with the control treatment during the whole experimental time. However, the CAT activity in gills was activated significantly (P<0.01) with the concentration of 5.38 g/L, and inhibited significantly (P<0.05) with the concentration of 10.51 g/L. The MDA contents in gills of Zebrafish enhanced gradually with the deicing salt concentration increased. In muscle tissues of Zebrafish, MDA contents decreased gradually during the whole experimental time.

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152-157

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February 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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