A SERRS Method for Coomassie Brilliant Blue Using Nanogold Aggregation Sol as Sunstrate

Abstract:

In pH 6.6 Na₂HPO₄-NaH₂PO₄ buffer solution and in the presence of NaCl, nanogolds were aggregated to form stable aggregated-nanogolds (ANG). The molecular probe of coomassie brilliant blue G-250 (CBBG) adsorbed on the surface of ANG to form CBBG -ANG conjugates that produce the strongest surface-enhanced resonance Raman scattering (SERRS) peak at 1171 cm^-1. Under the optimized conditions, the increased intensity ΔI was linear to CBBG concentration in the range of 0.29×10^-7 - 4.68×10^-7 mol/L, with the regression equation of ΔI = 0.86C - 17.1. A fast and sensitive method for trace CBBG was developed.

• Periodical: Advanced Materials Research (Volume 664)
• Pages: 731-735
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.664.731
• Online since: February 2013
• Authors: Gui Qing Wen, Duo Yang, Zhi Liang Jiang
• Keywords: Coomassie Brilliant Blue G-250, Nanogold, Surface-Enhanced Raman Scattering

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