An Enzyme-Catalytic Chemiluminescence Method for Detection of Ethanol Using HAuCl4-H2O2-Luminol System

Li Sheng Wang; Peng Fei Wang; Shan Shan Huang; Zhi Liang Jiang

doi:10.4028/www.scientific.net/AMR.668.153

Paper Titles

Degradation of Indoor Ammonia Using TiO₂ Thin Film Doped with Iron(III) under Visible Light Illumination
p.136

The Study on the Treatment of Nitrobenzene Wastewater by Heterogenious Catalysts and Electrode Oxidation
p.140

Synthesis of Copper Complexes of Poly [2-(dimethylamino ethyl methacrylate)-B-Poly (oligo (ethylene glycol) monomethylether methacrylate-B-Poly [2-(dimethylamino ethyl methacrylate)
p.145

Preparation of Cryolite from Industrial Grade Sodium Fluorosilicate
p.149

An Enzyme-Catalytic Chemiluminescence Method for Detection of Ethanol Using HAuCl₄-H₂O₂-Luminol System
p.153

Biodiesel Production through Direct Transesterification of Microalgae with Alcohol via Ultrasonic Wave
p.157

Investigation of Potentiostatic Electrodeposition of Ir in LiCl-KCl Molten Salt
p.162

Emission Characteristics of Particulate Number in an On-Board Test of a Diesel Passenger Car Fueled with Biodiesel
p.167

Investigation of the Solubility and Absorption Rate of CO₂ in DEA Promoted K₂CO₃ Aqueous Solution
p.174

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 668An Enzyme-Catalytic Chemiluminescence Method for...

An Enzyme-Catalytic Chemiluminescence Method for Detection of Ethanol Using HAuCl₄-H₂O₂-Luminol System

Abstract:

In pH 8.4 Tris-HCl buffer solutions, the alcohol dehydrogenase (ADH) catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide (NAD) to produce acetaldehyde. The acetaldehyde reduce HAuCl4 to form gold particles, and the excess HAuCl4 have enhancement effect on the chemiluminescence reaction of luminol-H2O2. The excess HAuCl4 decreased when ethanol increased, and the chemiluminescence value decreased. Accordingly we can utilize the chemiluminescence to detect the content of ethanol indirectly. The decreased chemiluminescence value (ΔI) was proportional to the ethanol concentration (C) from 0.017 to 5.10 mmol/L, with a regression equation of ΔI = 2374.3C+280.1, and a detection limit of 2.3 μmol/L. This proposed method was applied to detect ethanol in saliva samples of drunk, with satisfactory results