Effect of MgSO4, NaHCO3, KH2PO4, K2HPO4 and Ascorbic Acid on Survival of Bifidobacterium bifidum during Freeze-Drying

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Effect of MgSO4, NaHCO3, KH2PO4, K2HPO4 and ascorbic acid on the survival of Bifidobacterium bifidum and the number of viable cells of lyophilized powder was studied. Results were as follows: addition of MgSO4, NaHCO3, KH2PO4, K2HPO4 and ascorbic acid into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of MgSO4, NaHCO3, KH2PO4 , K2HPO4 and ascorbic acid were 0.20%, 0.60%, 1.80%, 1.40% and 2.20, the survival of B. bifidum was 78.31%, 77.92%, 77.80% ,79.82% and 71.62, respectively.

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346-349

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March 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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