Asymmetric Synthesis of Ethyl (R)-4-Chloro-3-Hydroxybutanoate with a Two Recombinant Escherichia coli System

Abstract:

The asymmetric reduction of ethyl 4-chloro-acetoacetate (CAAE) to ethyl (R)-4-chloro-3- hydroxybutanoate (R-CHBE) biocatalysed by the aldehyde reductase of Sporobolomyces salmonicolor expressed in E. coli M15 (pQE30-alr) in combination with regeneration of NADPH by the glucose dehydrogenase of Bacillus megaterium expressed in E. coli M15 (pQE30-gdh) was reported. The bioreduction was carried out in a two-phase reaction system with n-butyl acetate as an organic solvent. Bioconversion of 300 mmol CAAE with a final yield of 97.5 % and an enantiometric excess of 99 % was achieved without the addition of cofactor NADPH.

• Periodical: Advanced Materials Research (Volumes 690-693)
• Pages: 1188-1192
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.690-693.1188
• Online since: May 2013
• Authors: Ke Ju Jing, Wen Ting Ban
• Keywords: Aqueous-Organic Biphasic System, Asymmetric Reduction, Ethyl (R)-4-Chloro-3-Hydroxybutanoate, Two-Strain System

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