Prokaryotic Expression and Purification of Tomato Cnr Protein

Abstract:

Tomato fruit ripening mutant colorless non-ripening (Cnr) is an autosomal dominant mutant, obtained by natural mutation. The fruit of Cnr mutant was obviously immature, and the softening and lycopene accumulation of the Cnr fruit was significantly inhibited during the fruit ripening. In this work, we cloned the Cnr gene from the red ripe tomato fruit by RT-PCR, and constructed a prokaryotic expression vector. And the recombinant Cnr protein was induced by 1 mM IPTG in Escherichia coli BL21 (DE3), and purified by MBP affinity chromatography.

• Periodical: Advanced Materials Research (Volumes 690-693)
• Pages: 1247-1251
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.690-693.1247
• Online since: May 2013
• Authors: Yuan Hong Xie, Hui Liu, Hong Xing Zhang
• Keywords: Affinity Chromatography, Colorless Non-Ripening, Prokaryotic Expression, Tomato

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