Made Polypeptide from Sipunculs Nudus by Papain Enzymolysis and its Effects on Hydroxy Radical Cleaning

Yin Ling Zhu; Si Dong Li; Yan Liao; Jiang Ming Li

doi:10.4028/www.scientific.net/AMR.699.360

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 699Made Polypeptide from Sipunculs Nudus by Papain...

Made Polypeptide from Sipunculs Nudus by Papain Enzymolysis and its Effects on Hydroxy Radical Cleaning

Abstract:

The polypeptide of Sipunculs nudus was prepared by papain. The optimum papain enzymolysis process to Sipunculs nudus was confirmed by means of single factor analysis and orthogonal test, it showed as below: 300U/g of enzyme addition amount, 50°C of temperature, 60min of enzymolysis time and pH7.0. The clearance rate of hydroxy radical is 95.42% according to the optimum process on Sipunculs nudus enzymolysis. The HPLC chromatogram showed that the prepared component of antioxidative active polypeptide according to such condition is single and its molecular weight is 5868. The results of experiment shows that Sipunculs nudus polypeptide have high inoxidizability and high removing free radicals ability.

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Periodical:

Advanced Materials Research (Volume 699)

Pages:

360-366

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.699.360

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Online since:

May 2013

Authors:

Yin Ling Zhu, Si Dong Li, Yan Liao, Jiang Ming Li

Keywords:

Hydroxy Radical Cleaning, Papain, Polypeptide, Sipunculs nudus

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