Advanced Materials Research Vol. 699

Abstract: A selective cancer cell death induced by discharged hydrogen from Pd-Ni base hydrogen storage alloy (HSA) powder is demonstrated. From the cell experiment and the measurement of surviving cell rate, it was found that cell death was seen only in cancer cells (HeLa : cervical cancer cell) after 24h- and 48h-immersion of Pd-5at.%Ni alloy powder, while no harmful influence was observed in normal cells (MDCK : renal epithelial cell). The range of cancer cell death was limited just near the HSA powder. It is suggested that the hydrogen radical emitted from the HSA powder induces the formation of hydrogen peroxide and brings a detrimental effect in the cancer cell, while hydrogen peroxide is decomposed by the enzyme, catalase, in normal cells, resulting in the selective death of cancer cells.

Abstract: In order to improve beer sterilization effect of wastewater treatment, reduce the heat load of the sterilization wastewater on subsequent water treatment processes. In this paper, through optimizing transformation developed micro-flocculation -membrane treatment processes. Screening for the identification of inorganic polymeric aluminum ferric chloride flocculants as the technology. The best dosage was 15mg / L, the field test to verify the craft beer sterilized wastewater treatment effect. The results show that, micro flocculation membrane treatment process in the water after treatment of COD and turbidity removal rate is respectively 76% and 98%. To illustrate the use of this technology in the treatment of beer sterilizing water without two stage filtration, can effectively recycling sterilized wastewater.

Abstract: The mechanism of cancer cell death induced by hydrogen discharged from Pd-5at.% Ni hydrogen storage alloy has been investigated. Cancer cell (HeLa : cervical cancer cell) death was observed in the limited region within ~ 3 mm from the sample. The measurement of surviving fraction of cells revealed that almost all the cancer cells in the well of 96-well multi plate, 6.2 mm in diameter were extinct (p < 0.01), while no detectable influence was observed in the normal cells. From the fluorescent imaging experiment, it was indicated that the cell death induced by discharged hydrogen was due to the “Apoptosis” and hydrogen peroxide was detected in both intracellular and extracellular regions. Furthermore, the generation of hydrogen radical and hydroxyl radical was observed in the ESR measurement. From the results obtained, the mechanism of cancer cell death is proposed.

Abstract: .Membrane bioreactor (MBR) is considered to be a promising technology for combine biological with filtration and many advantages over conventional activated sludge (CAS) processes. In this study, the submerged membrane bioreactor which was explored by our research group and JDL Environmental Protection Ltd. at condition of aerobic-MBR and SBR-MBR were investigated. Hydraulic characteristics of MBR by the tracer experiments showed the total dead space (Vd, %) in the MBR was 3.2%. Compared with aerobic-MBR situation, the SBR-MBR exhibited better performance of average NH4+-N, total nitrogen, and phosphorus removal efficiency 90%, 86%, 57% respectively. The content of protein was correlated to sludge activity and organic matter. The initial membrane fouling was caused by layer pollution and subsequently the extra-cellular polymeric substances (EPS) contributed.

Abstract: The goal in this paper is to investigate the efficiency of poly-PLL (Poly-L-lysine)/ Alg (Alginate) vector mediated virus genomic DNA transfection and the virus genomic DNA’s biological activity in vivo. After Pseudorabies virus (PRV) genomic DNA being adhered to the porous СаСО3 particles, PLL and Alg were alternately polymerized on the surface of the porous DNA-СаСО₃ particles to 7 layers, which were later dissolved them by EDTA to remove СаСО₃ cores; the vectors in which the DNA were coated by poly-PLL/Alg, were harvested to infect the rabbits and observe the replication of viral DNA. Porous СаСО3 particles, which were obtained from the reaction between Na2CO₃ and CaCl₂, had an efficiency of absorbing DNA 1 µg/mg СаСО₃ particles. After being coated by PLL/Alg, microcapsules were obtained with the diameter of 2-4 µm. 10.0 µg of poly-PLL/Alg-PRV DNA microcapsules could cause rabbits’ death by intramuscular injection. The identification of PCR shows that the death was caused by PRV infection. The results indicate that Poly-PLL/Alg microcapsules can mediate efficient transfection of DNA.

Abstract: The α-Fe₂O₃ nanotubes were prepared by the eletrospinning and calcination process. The as-prepared α-Fe₂O₃ nanotubes were characterized by XRD, SEM and BET. The XRD patterns show that the nanotubes with a pure hematite phase (α-Fe₂O₃) are obtained after calcination at 550 oC. The SEM morphologies and BET measurement demonstrate the nanotubes have an average diameter of 200-300 nm and a specific surface area of 15.1 m2/g. Their adsorption of methyl blue was studied with ultraviolet spectrophotometer (UV) to measure the concentration of methyl blue in aqueous solution, and the adsorption kinetics is basically in agreement with the pseudo-second-order kinetic model in the methyl blue concentration range of 100-300 mg/L.

Abstract: This paper presents the effect of nitrogen concentration (C_N) on aluminum-nitride bonding formation, structure and morphology of the aluminum nitride films. The films on the unheated substrates were deposited by radio frequency reactive magnetron sputtering technology using an aluminum target under argon/nitrogen mixture atmosphere. The FTIR and Raman spectra of the films confirmed their absorption bands corresponding to E₁ (TO), A₁ (TO), and E₂ (high) vibration modes of the infrared active aluminum-nitride bonding. The crystallographic orientation of the films was optimized under C_N of 40%. The cross-sectional FE-SEM image of the film under this condition showed the columnar structure. The dense columnar grains were uniformly observed on the films surface under all C_N, except for C_N of 20%. The bulk resistivity and piezoelectric property were investigated via the metal-insulator-metal structures. The results showed that the resistivity was in a range of 10¹⁴-10¹⁵ Ωcm while the effective piezoelectric coefficient was 11.03 pm/V.

Abstract: To study the concentration of anammox process in the biofilter .Making ANAMMOX biofilter as the reactor, control the reaction water temperature at 25~31°C,increasing the influent concention of NH4+-N and NO2--N gradually to study the rule of denitrification of ANAMMOX biological filter layers in different concentrations load variation. ANAMMOX biofilter filter layer biomass distribution after the frist increase and then decrease gradually, the last flatten the trend. The biomass of ANAMMOX is concentrated in the 0~50cm filter layer and inlet at low biomass.The denitrification performance of ANAMMOX best at the concentration of NH4+-N and NO2--N 90 mg/L,120mg/L, but will be greater inhibited at the concentration of NH4+-N and NO2--N higher than 120 mg/L,160mg/L.

Abstract: Antibacterial ability of brewer's spent grains peptides was detected which were hydrolyzed in different hydrolysis time with four proteases. The results showed: Flavourzyme hydrolyze in 180 min has better antibacterial ability to Staphylococcus aureus. The hydrolyzate was separated with polyamide column chromatography, ion exchange column chromatography, respectively. Peptides were tested of antibacterial ability, and better antibacterial ability were collected. Molecular weight is about 1877.67 which is measured with gel column chromatography. The minimal inhibitory concentration to Staphylococcus aureus was 2%.

Abstract: Combustion control techniques have become a legal requirement to minimize pollution in municipal solid waste incinerators. Typically, incinerator destruction of pollutants is achieved when 2-second gas residence time at 8500 Celsius and about 6% O2 are guaranteed at exit. Performance of a fixed bed (two-stage) thermal oxidizer for solid waste is analyzed numerically using computational fluid dynamics (CFD) technique. The CFD analysis provides three-dimensional view of thermal and gas flow field inside the thermal oxidizer chamber. Localized zones of temperature and species concentration were analyzed and provided critical information for understanding the thermo-chemical processes taking place during incineration leading into design optimization and the operation strategy of the thermal oxidizer. Based on the CFD results, the original design of the thermal oxidizer was modified to optimize the flow characteristics and the residence time in the secondary chamber thereby achieving complete combustion of gases emanating from the lower chamber, hence less emissions of CO.