Self-Assembled Au Electrode for Direct Electrochemistry of Horseradish Peroxidase and Detection of Hydrogen Peroxide

Abstract:

The H₂O₂ biosensor was prepared by continuous processes: 2,3-dimercaptosuccinic acid (DMSA) self-assembly monolayers gold nanoparticles (AuNPs) film by electrodepositioncovalent immobilization of horseradish peroxidase (HRP). In pH 7.2 phosphoric buffer solution (PBS), the HRP-AuNPs-DMSA-Au electrode exhibited a pair of well-defined cyclic voltammetric peaks in H₂O₂ solution, and the oxidation peak is about +0.4V versus saturated calomel electrode (SCE). The resulting substrates were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor exhibited remarkable sensitivity towards H₂O₂ and a wide dynamic range of 2-800 μM. The electrode retained 90% of its initial activity after 30 days of storage at 4°C.

• Periodical: Advanced Materials Research (Volume 704)
• Pages: 72-76
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.704.72
• Online since: June 2013
• Authors: Han Cui, Zhao Hao Wang, Qi Jin Wan, Nian Jun Yang
• Keywords: Gold Nanoparticles (GNP), H₂O₂ Catalytic Oxidation, Horseradish Peroxidase (HRP), Self-Assembled Au Electrode

References

[1] C. Leger, P. Bertrand, Chem. Rev. 108 (2008) 2379-2438.

[2] Y. Liu, M.K. Wang, F. Zhao, Z.A. Xu, S.J. Dong, Biosens. Bioelectron. 21 (2005) 984-988.

[3] H. Ohshima, M. Tatemichi, T. Sawa, Arch. Biochem. Biophys. 417 (2003) 3-11.

[4] R. Villalonga, P. Díez, P. Yáñez-Sedeño, J.M. Pingarrón, Electrochimica. Acta. 56 (2011) 4672-4677.

DOI: 10.1016/j.electacta.2011.02.108

[5] M. Monica, Mat. Sci. Eng. C. 22 (2002) 449-452.

[6] Y. Liu, F. Yin, Y. Long, Z. Zhang, S. Yao, Colloid Interface Sci. 258 (2003) 75-81.

[7] N. Yang, X. Wang, Q. Wan, Electrochimica. Acta. 52 (2007) 4818-4824.

[8] X. Luo, J. Xu, H.Y. Chen, Biosens. Bioelectron. 21 (2005) 190-196.

[9] S.R. Sainkar, A.B. Mandale, M. Rao, M. Sastry, Langmuir. 17 (2001) 1674-1679.

[10] A. Gole, S. Vyas, S. Phadtare, A. Lachke, M. Sastry, Colloid Surf. B. 25 (2002) 129-138.

[11] H. Yin, W.S. Ai, L. Shi, S. Zhu, Sensors and Actuators B Chem. 137 (2009) 747-752.

[12] R. Villalonga, P. Díez, P. Yáñez-Sedeño, J.M. Pingarrón, Electrochimica. Acta. 56 (2011) 4672-4677.

DOI: 10.1016/j.electacta.2011.02.108

[13] M.C. Y. Chang, A. Pralle, E.Y. Isacoff, C.J. Chang, Am. Chem. Soc. 126 (2004) 15392-15393.

DOI: 10.1021/ja0441716

[14] A. Sevanian, P. Hochstein, Annu. Rev. Nutr. 5 (1985) 365-390.

[15] W. Troll, R. Wiesner, Annu. Rev. Pharmacol. Toxicol. 25 (1985) 509-528.