Preparation of GR/HRP/Chit Modified Electrode and its Electrochemical Behaviors

Abstract:

Horseradish peroxidase (HRP) was immobilized on the glassy carbon electrode surface by using GR, and GR/HRP/chit modified electrode was prepared. The electrochemical behaviors of GR/HRP/chit/GCE were studied by many electrochemical methods. Immobilized HRP showed a pair of stable redox waves, and oxidation peak was very small, the reduction of peak potential for 0.33 V vs. SCE and retained its bioelectrocatalytic activity for the reduction of H₂O₂. At the same time, a series of related parameters of the enzyme electrical activity were calculated, further experimental results reflected the enzyme mechanism in the process of reaction.

• Periodical: Advanced Materials Research (Volume 704)
• Pages: 87-91
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.704.87
• Online since: June 2013
• Authors: Hao Shu, Yan Li Shang, Han Cui, Qi Jin Wan, Nian Jun Yang
• Keywords: Direct Electron Transfer, Graphene (GR), Horseradish Peroxidase (HRP)

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