Comparison on Antimicrobial Activity of Different Solvent Extracts from Aerial Stem and Rhizome of Douttuynia cordata

Abstract:

The 95% of ethanol crude extract of aerial stem and rhizome of houttuynia cordata was sequentially extracted with petroleum ether, ethyl acetate, n-butyl alcohol and water. Antimicrobial activity and minimum inhibitory concentration (MIC) of the different extracts against Staphylococlus aureus, Escherichia coli, Bacillus subtilis, Aspergillus niger and Saccharomycetes were examined with filter paper method. And antimicrobial activity between aerial stem and rhizome was compared. The result showed that, among the extracts of aerial stem and rhizome of houttuynia cordata, with regard to antimicrobial activity, water extract was strongest against Staphylococlus aureus, and ethyl acetate extract was strongest against Escherichia coli, and n-butyl alcohol extract was strongest against Bacillus subtilis and Saccharomycetes, while each extract had no significant antimicrobial activity against Aspergillus niger. Moreover, the antimicrobial activity of rhizome was stronger than that of the aerial stem.

• Periodical: Advanced Materials Research (Volumes 706-708)
• Pages: 44-47
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.706-708.44
• Online since: June 2013
• Authors: Shao Juan Jiang
• Keywords: Antimicrobial Activity, Douttuynia cordata, Filter Paper Method, Minimum Inhibitory Concentration

References

[1] Yongqian Cheng, Flora of China[M], Beijing: Science Press,1982:8.

[2] Sen Yao, Jiuyao Zhou, Pharmacology and clinical application of houttuynia cordata[J], LISHIAHEN MEDICINE AND MATERIA MEDICA RESEARCH, 2008,19(1):237-240.

[3] Xiumei Ji, Liping Ma, Research progress of houttuynia cordata[J], JOURNAL OF HENAN MEDICAL COLLEGE FOR STAFF AND WORKERS, 2007, 19(1):91-93.

[4] Jing Li, Yuan Ma, Comparative study on bacteria inhibitive effect of alcohol extract from Hortuyia cordala Thund in vitro[J], Jiangsu Agricultural Sciences, 2011(1):373-374.

[5] Ying Zhng, Binger Zheng, Study on extraction conditions and antimicrobial activity of antibacterial materials from Houttuyniae cordata[J], Guangdong Agricultural Sciences, 2011(20): 91-93.

[6] ZHANG Ying, Study on the content and the bacteria-suppression of houttuynia cordatu thunb beverage[J], FOOD SCIENCE AND TECHNOLOGY, 2008,33(11):110-112.

[7] WANG Jian, SHI Yu, ZHANG Yong-ze, LI Qing-mei, MEN Jin-e, Research of anti- biotic and anti- virus activities on extraction from different parts of Houttuynia cordata[J], JOURNAL OF HEBEI UNIVERSITY OF ENGINEERING(NATURAL SCIENCE EDITION), 2010,27(2): 104-106.

[8] ZHOU Guo-hai, YU Hua-zhong, YU Yong-hong, Study on bacteriostatic action in different parts of Houttuynia cordata[J], HUNAN FORESTRY SCIENCE & TECHNOLOGY, 2006,33(6): 38-40.

[9] Ping Shen, Xiurong Fan, Guanwu Li. microbiology experiment[M], Beijing: High Education Press, 2006:50-54.

[10] Lijuan Cheng, Quanhong Xue, experimental technique in microbiology[M], Xi'an: World Publishing Corporation, 2000:108.

[11] WANG Rui-jun, In vitro Antibacterial Effect of Several Common Chinese Medicinal Herbs on Staphlococcus aureus[J], MODERN FOOD SCIENCE & TECHNOLOGY, 2009, 25(9): 1104-1106.