Study on the Conditions of Straw Powder Degradation to Produce Reducing Sugar in Mixed Cultivation

Abstract:

A strain of trichoderma and aspergillus filtered out from the environment was used to ferment in powder-liquid medium where the main carbon source was rice straw powder. Along with the fermentation, the pretreated rice straw powder was catalytic degraded into reducing sugar by enzyme. There was orthogonal test based on single factor experiment. The orthogonal experiment showed that the crude enzyme liquid dosage caused the biggest influence on the degradation of straw powder, second was temperature and followed by pH value, and the straw powder concentration caused the minimum effects on degradation. The optimal conditions of degradation of rice straw powder was: the percentage of the volume of crude enzyme liquid in total degradation liquid was 3%, initial straw powder concentration was 60g/L, the pH value was 4.8, and the degradation temperature was 50°C. Under the optimal conditions, the degradation rate could be able to reach 80%.

• Periodical: Advanced Materials Research (Volumes 724-725)
• Pages: 306-309
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.724-725.306
• Online since: August 2013
• Authors: Yi Liu, Yue Hua Yuan
• Keywords: Cellulose, Degradation, Mixed Cultivation, Reducing Sugar, Rice Straw Powder

References

[1] Gray K A, Zhao L H: Current Opinion in Chemical Biology, Vol. 10.2 (2006), pp.141-146.

[2] Li S Z: Science & Technology Review, Vol.23 (5) (2005), pp.9-11.

[3] Selig, M J, Knoshaug, E P, Adney, W S: Bioresource Technology, Vol. 99(11) (2008), pp.4997-5005.

DOI: 10.1016/j.biortech.2007.09.064

[4] Rajeev Kumar, Wyan, C E: Bioresource Technology, Vol. 100(18) (2009), pp.4203-4213.

[5] Zhang QZ, He GF, Wang J: Biomass and Bioenergy, Vol. 33(10)(2009), pp.1464-1468.

[6] Zhu D S, Wu Y X, Yu Z N, Li H, Zhang X:Chemestry and Industry of Forest Products, Vol.25(1) (2005),pp.112-114.

[7] Liu Y, Yuan Y H: Jiangsu Agricultural Sciences, Vol.40 (5) (2012), pp.306-310.

[8] Yao R S, Deng S S, Qi B K, Yu Y:Fine Chemicals,Vol.25(2) (2008),pp.155-158

[9] Li Y Y, Hu C R: Experiment Design and Data Processing, pressed by Chemical Industry Press (2005), pp.79-90.

[10] Zhang L X, Zhang T F, Li L Y: Biochemical experiment method and technology, pressed by Higher Education Press (2001), pp.1-3.

[11] Gregg D J, Saddler J N: Biotechnology and Bioengineering, Vol. 5l (4) (1996), pp.375-383.

[12] Andreas Rudolf, Malek Alkasrawi, Guido Zacchi: Enzyme and Microbial Technology, Vol. 37 (2) (2005), pp.195-204.