Optimization of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Cotton Stalk

Abstract:

Steam-exploded cotton stalk was used as raw material in ethanol production through simultaneous saccharification and fermentation by Penicillium Q59 and Saccharomyces cerevisiae P1. The fermentative conditions were firstly examined by single factor experiments to determine the central point in Box-Behnken design, which was explored with expectation to get optimized fermentative conditions for enhancement of ethanol production. The results of optimized fermentative conditions were determined as follows: fermentation time was 10.5 days, bran added percent was 15%, initial pH value was 5.5. Under the optimal conditions, the experimental yield of ethanol was 99.85 ± 4.21 g·kg^-1SECS (steam-exploded cotton stalk), which was close to the theoretical predicting value, it showed the model was feasible. The research results will provide technical reference for further exploitation of cotton stalk.

• Periodical: Advanced Materials Research (Volumes 724-725)
• Pages: 391-398
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.724-725.391
• Online since: August 2013
• Authors: Qin Zhang, Yan Bin Li, Zhan Wen Liu, Yun Feng Pu, Li Ming Xia
• Keywords: Ethanol, Optimization, Response Surface Methodology (RSM), Simultaneous Saccharification, Simultaneous Saccharification and Fermentation (SSF)

References

[1] J. Shi, R. S. Ratna, C. Mari and H. Noura: Biomass and Bioenergy Vol. 33 (2009), pp.88-96

[2] Q. Zhang, Y. B. Li and Z. H. He: Acta Agriculturae Boreali-occidentalis Sinica Vol. 20 (2011), pp.194-198 (In Chinese)

[3] A. Isci and G. N. Demirer: Renewable Energy Vol. 32 (2007), pp.750-757

[4] Q. Zhang, Y. B Li and Y. L. Cao: Food Science, Vol. 32 (2011), pp.192-197 (In Chinese)

[5] K. Ohgren, A. Rudolf, M. Galbe and G. Zacchi: Biomass and Bioenergy Vol. 30 (2006), pp.863-869

DOI: 10.1016/j.biombioe.2006.02.002

[6] R. Lahlali, S. Massart, M. N. Serrhini and M. H. Jijakli: International Journal of Food Microbiology, Vol. 122 (2008), pp.100-108

[7] W. J. Gong, Y. P. Zhang, G. R. Xu, X. J. Wei and K. P. Lee: Journal of Central South University Technology, Vol. 14 (2007), pp.196-201 (In Chinese)

[8] A. Singh and N. R. Bishnoi: Industrial Crops and Products Vol. 37 (2012), pp.334-341

[9] L. J. Wang, Z. L. Luo and A. Shahbazi: Industrial Crops and Products Vol. 42 (2013), pp.280-291

[10] Z. L. Lai, C Chang, X. J. Ma, W. Wei, X. S. Ma and D. Wang: Transactions of the CSAE Vol. 26 (2010), pp.250-254 (In Chinese)

[11] P. Luo, Z. Liu, C. M. Yang and G. S. Wang: Chemical Engineering Vol. 35 (2007), pp.42-45 (In Chinese)

[12] Y. B. Li, Q. Zhang, Y. F. Yue and M. F. Gong: Liquor-Making Science & Technology, (2009), pp.121-123 (In Chinese)

[13] Q. Zhang, Y. B. Li, J. J. Li and C. M. Ma: Proceedings 2011 International Conference on Materials for Renewable Energy & Environment, (2011), pp.459-463

[14] G. Miller: Analytical Chemistry Vol. 31 (1959), pp.426-428

[15] H. B. Seo, H. J. Kim, O. K. Lee, J. H. Ha, H. Y. Lee and K. H. Jung: Journal of Industrial Microbiology & Biotechnology Vol. 36 (2009), pp.285-292

[16] P. J. Van Soest, J. B. Robertson and B. A. Lewis: Journal of Dairy Science Vol. 74 (1991), pp.3583-3597

[17] J. M. Zhang, A. Gengb, C. Y. Yao, Y. H. Lu and Q. B. Li: Bioresource Technology Vol. 105 (2012), pp.134-141

[18] T. Sugimoto, T. Makita, K. Watanabe and H. Shoji: Journal of Industrial Microbiology and Biotechnology Vol. 39 (2012), pp.605-612

[19] Q. Zhang, Y. Fu, Y. Wang, J. Han, J. Lv and S. Wang: Journal of Applied Microbiology, Vol. 112 (2011), pp.280-288

[20] E. C. Rivera, A. C. Costa, D. I. P. Atala, F. Maugeri, M. R. W. Maciel and R. M. Filho: Process Biochemistry Vol. 41 (2006), pp.1682-1687

[21] Y. L. Bao, L. Chen, Z. C.Yan and H. L. Wang: Antonie van Leeuwenhoek Vol. 99 (2011), pp.329-339

[22] E. Silva, H. Rogez and Y. Larondelle: Separation and Purification Technology Vol. 55 (2007), pp.381-387

[23] K. Sinha, P. D. Saha and S. Datta: Industrial Crop Production Vol. 37 (2012), pp.408-414

[24] R. V.Muralidhar, R. R. Chirumamila, R. Marchant and P. Nigam: Biochemistry Engineering Journal Vol. 9 (2001), pp.17-23