Effect of Acetic Acid on Ethanol Fermentation by Engineered Escherichia coli SZ470

Abstract:

Hydrolysis of the aquatic vegetables waste would lead to the generation of some toxic components and by-products, such as weak acids, aldehydes and phenols. They might do harm to sequent fermentation process to some extent. The toxic effect of acetic acid on ethanol fermentation by ethanologenic Escherichia coli SZ470 was investigated. The growth curves of Escherichia coli SZ470, specific growth rate (μ), the ethanol yield and consumption of glucose were compared with different concentrations of acetic acid addition in the fermentation medium. When concentrations of acetic acid exceed 0.8 g/L, the exponential growth phase of Escherichia coli SZ470 was significantly expanded from 10 h to above 12 h, moreover, the ethanol yield and consumption of glucose drastically decreased. Specific growth rate increased as acetic acid concentrations below 0.6 g/L, but fell as acetic acid concentrations exceeded 0.6 g/L, the result indicated that minor amount of acetic acid might be helpful with growth of Escherichia coli SZ470.

• Periodical: Advanced Materials Research (Volumes 724-725)
• Pages: 369-372
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.724-725.369
• Online since: August 2013
• Authors: Jin Hua Wang, Feng Huang, Xiao Zhao, Jin Fang Zhao, Yong Ze Wang, Sheng De Zhou
• Keywords: Acetic Acid, Ethanol Fermentation

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