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A Novel Microbial Biosensor for Toxicity Test
Abstract:
A mercury chloride sensitive Bacillus sp. X4 as a toxicity recognition element was isolated from soil. A novel microbial biosensor was developed based on the isolated X4 in combination with a dissolved oxygen electrode for the analysis of toxicity in wastewater. Optimal response of the biosensor was obtained at pH 7.0 - 7.5 and 30 - 35 °C with a detection linearity over the range 0.10 - 1.97 mg·L-1 of HgCl2. The repeatability of the sensor response were found to be 1.8%. Preliminary application of the biosensor in pharmacy wastewaters has demonstrated their effectiveness. Toxicity values produced with this biosensor for pharmacy wastewaters correlated well with those determined by standard method.
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1204-1208
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August 2013
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© 2013 Trans Tech Publications Ltd. All Rights Reserved
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