A Comparative Study on the Analysis Methods for Chlorophyll-a

Abstract:

This paper investigated three chlorophyll-a analysis methods in water sample. The UV-VIS spectrophotometry method followed SL88-2012 standard procedure, the results showed the detection limit of chlorophyll-a was 0.22 μg/L, with the lower detection limit of 1.0 μg/L. The detection range of the vivo chlorophyll-a fluorescence method was 0.1-300μg/L, the ratios of different algae matched that prepared in water samples. The detection limits and quantification limits for HPLC by using UVD and FLD were respectively 0.013 and 0.004 μg/L, 0.040 and 0.013μg/L. FLD was more sensitive than UVD for chlorophyll-a analysis, and the recovery of standard addition was in the range of 90.2% to 103.9% with UVD and FLD. Given the different pretreatment procedures of the sample, equipment conditions, correction and precision, vivo chlorophyll-a fluorescence method was the best on-site monitoring and the emergency monitoring method. Spectrophotometer was suitable for routine laboratory determination of chlorophyll-a, especially for bulk water samples. HPLC method had high precision and sensitivity, so this method applied to the determination of exact requirements, microanalysis and pigment separation.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 1411-1415
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.1411
• Online since: August 2013
• Authors: Fei Peng*, Shi Bo Liu, Hai Yan Xu, Zhi Qun Li
• Keywords: Chlorophyll-a, High Performance Liquid Chromatography (HPLC), In Vivo Chlorophyll Fluorescence, Spectrophotometry

References

[1] J W. LaBaugh, Relation of algal biovolume to chl-a in selected lakes and wetlands in north-central United States[J]. Canadian Journal of Fisheries and Aquatic Sciences, 1995(52): 416~424.

DOI: 10.1139/f95-043

[2] B. Freedman Environmental Ecology[B]. Sandiego Academic Press, 2002.

[3] K.H. Wiltshire, S. Harsdorf, B. Smidt, G. Blocker, R. Reuter and F. Schroeder. The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary and the German Bight: A comparison of high-performance liquid chromatography, delayed-uorescence and prompt fluorescence methods[J]. Journal of Experimental Marine Biology and Ecology, 1998(222): 113-131

DOI: 10.1016/s0022-0981(97)00141-x

[4] Wang Ming-cui, Liu Xue-qing, Zhang Jian-hui. Evaluate method and classification standard on lake eutrophication[J]. Environmental Monitoring in China, 2002,18(5):47~49.

[5] P. Eullaffroy and Guy Vernet. The F684/F735 chlorophyll fluorescence ratio: a potential tool for rapid detection and determination of herbicide phytotoxicity in algae[J]. Water Research, 2003(37): 1983~1990.

DOI: 10.1016/s0043-1354(02)00621-8

[6] M.F. Fernandez-Leon, M. Lozano, M.C. Ayuso, A.M. Fernandez-Leon and D. Gonzalez-Gomez. Fast and accurate alternative UV-chemometric method for the determination of chlorophyll A and B in broccoli (Brassica oleracea Italica) and cabbage (Brassica oleracea Sabauda) plants[J]. Journal of Food Composition and Analysis.2010 (23): 809-813.

DOI: 10.1016/j.jfca.2010.03.024

[7] L. Moberg, G. Robertsson and B. Karlberg. Spectrofluorimetric determination of chlorophylls and pheopigments using parallel factor analysis[J]. Talanta. 2001 (54): 161-170.

DOI: 10.1016/s0039-9140(00)00650-0

[8] S.C. Huang, C.F. Hung, W.B. Wu, B.H. Chen. Determination of chlorophylls and their derivatives in Gynostemma pentaphyllum Malcino by liquid chromatography-mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis. 2008 (48): 105-112.

DOI: 10.1016/j.jpba.2008.05.009

[9] Water quality-Determination of Chlorophyll by Spectrophotometry (SL88-2012)[S].

[10] Feng Jing, Li Yanbo, Zhu Qing and Wu Weizhong. Comparision of methods for phytoplankton chlorophyll-a concentration measurement[J]. Ecology and Environment. 2008,17(2) :524-527.

[11] Gao Qi, Zhao Shuguang, Tan Yumei, Ultrasonic extraction spectrophotometric determination of chlorophyll content in the plant leeks Science and Technology of Food Industry[J] 2007,28(8): 166~167.

[12] P. Eullaffroy and G. Vernet. The F684/F735 chlorophyll fluorescence ratio: a potential tool for rapid detection and determination of herbicide phytotoxicity in algae[J]. Water Research. 2003 (37): 1983-1990.

DOI: 10.1016/s0043-1354(02)00621-8