Application of 16S rDNA Ribosomal RNA Approach for the Analysis and Identification of Bacterium in Granular Sludge

Yao Jie Deng; Wen Zhe Song; Yuan Cai Chen

doi:10.4028/www.scientific.net/AMR.726-731.255

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Application of 16S rDNA Ribosomal RNA Approach for...

Application of 16S rDNA Ribosomal RNA Approach for the Analysis and Identification of Bacterium in Granular Sludge

Abstract:

Both Eubacteria and Archaea community diversity and dynamicity were studied during PCP-degrading micro-aerobic granular sludge cultivation using 16S rDNA ribosomal RNA approach. Main bands of DGGE were compared with sequences. The results showed that Eubacteria and Archaea community were closely related to uncultured microorganisms. There were aerobic, micro-aerobic and anaerobic bacterium in micro-aerobic granular sludge simultaneously. After granular sludge was domesticated by PCP, the dominant Eubacteria and Archaea for PCP degradation were related to Proteobacteria, Sphingomonas, Methanogenic bacterium and so on.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

255-259

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.255

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Online since:

August 2013

Authors:

Yao Jie Deng, Wen Zhe Song, Yuan Cai Chen

Keywords:

16S rDNA Ribosomal RNA Approach, Micro-Aerobic Granules, Microbial Population, Pentachlorophenol (PCP)

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