Immobilization of Naringin onto Chitosan Substrates by Using Ozone Activation

Abstract:

The ozone oxidation can easily produce peroxides with free radicals for the surface modification on biomaterials. This process would be highly efficient and without toxicity. In this research, naringin, a HMG-CoA reductase inhibitor which can promote bone formation, was immobilize onto chitosan film by using the ozone activation process. At first, chitosan films were treated by the ozone activation to produce peroxides for the following immobilization of naringin. The amounts of peroxides produced by ozone treatment were quantified by the iodide assay. The immobilized naringin were identified with UV and FTIR. The results indicated successful immobilization of naringin. The concentration of crosslinkers was also optimized in this study. From SEM images, the surface topography of chitosan film was not changed after the immobilization process. The FTIR spectra indicated the difference in amine bonds of chitosan, revealing that there would be the chemical reaction between chitosan and crosslinkers. In the in vitro delivery, the chitosan substrate with immobilized naringin was immersed in PBS and the released amount of naringin was measured by UV every two days. It was found that the immobilized naringin was slowly released in two weeks, where the naringin concentration was successfully controlled by this delivery process. The results of cell culture showed that cell activity, attachment and proliferation were promoted with immobilized naringin without any cytotoxicity. The early osteoblastic differentiation, ALPase expression, was also enhanced. The results in this research demonstrated the successful immobilization of naringin onto chitosan substrates. With the slow delivery of naringin, the naringin-chitosan substrate was highly osteoconductive without cytoxicity.

• Periodical: Advanced Materials Research (Volume 747)
• Pages: 119-122
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.747.119
• Online since: August 2013
• Authors: Ming Hua Ho, Jing Wei Wang
• Keywords: Bone Tissue Engineering, Chitosan, Naringin, Osteoblast, Ozone, Surface Modification

References

[1] L.L. Chen, L.H. Lei, P.H. Ding, Q. Tang, Y.M. Wu, Osteogenic effect of Drynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model, Archives of Oral Biology 56 (2011) 1655-62.

DOI: 10.1016/j.archoralbio.2011.06.008

[2] R.W.K. Wong, A.B.M. Rabie , Bone induction using hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, Hong Kong Dental Journal, 4 (2007) 15-21.

[3] M. Geiger, R.H. Li, W. Friess, Collagen sponges for bone regeneration with rhBMP-2, Adv. Drug Deliv. Rev. 55 (2003) 1613–29.

DOI: 10.1016/j.addr.2003.08.010

[4] M.H. Ho, L.T. Hou, C.Y. Tu, H.J. Hsieh, J.Y. Lai, W.J. Chen, D.M. Wang, Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment, Macromol. Biosci. 26 (2006) 5617-23.

DOI: 10.1002/mabi.200500130

[5] M. H. Ho, L. T. Hou, C. Y. Tu, H. J. Hsieh, J. Y. Lai, W. J. Chen, D. M. Wang, Efficient. Modification on PLLA by Ozone Treatment for Biomedical Applications, Macromolecular bioscience 7 (2007) 467-74.

DOI: 10.1002/mabi.200600241