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Synthesis and Identification of Heavy Metal Lead Artificial Antigen

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Abstract:

Lead ions were coupled to protein carrier bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH) respectively. Diethylene Triamine Pentacetate Acid (DTPA) was used as bifunctional chelator. Pb-DTPA-Carrier protein, DTPA-Carrier protein and carrier protein were detected qualitatively with ICP-MS, UV spectrophotometer and electrophoresis. It turns out that the Pb-DTPA-Carrier protein contains certain concentration of heavy metal ion, the maximum ultraviolet absorption peak value of Pb-DTPA-Carrier protein significantly higher than that of DTPA-Carrier protein and carrier protein, and the electrophoresis reveals more vague bands of Pb-DTPA-Carrier protein with slower migrations speed comparing to the bands of the DTPA-Carrier protein and carrier protein. The result indicated that the artificial antigen for lead was successfully synthesized.

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Periodical:

Advanced Materials Research (Volumes 750-752)

Pages:

1405-1409

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.750-752.1405

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Online since:

August 2013

Authors:

Hai Qing Ye, Jing Bo Liu, Jun Xiao Wei, Meng Lei Xu, Yan Song Li

Keywords:

Artificial Antigen, Bifunctional Chelator, ICP-MS, Lead

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