Effect of Ionic Liquid Pretreatment on the Enzymatic Saccharification of Sugarcane Bagasse

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Abstract:

onic liquid, 1-n-butyl-3-methylimidazolium chloride ([C4mi Cl) was used to pretreat sugarcane bagasse (SCB), followed by regeneration of SCB by addition of deionized water. The regenerated SCB exhibited enhancement in enzymatic saccharification with an average 3.1-fold enhancement in yield of reducing sugar produced compared to untreated SCB in the kinetic study. The maximum yield of glucose and reducing sugar produced were 2.6% and 4.3% respectively for untreated SCB, whereas the maximum yield of glucose and reducing sugar produced were more significant with value of 7.2% and 16.6% respectively for IL-pretreated SCB. This paper suggested that the IL-pretreatment of SCB could be a useful pretreatment method to increase the yield of sugars produced in enzymatic saccharification.

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246-251

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September 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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[1] Ministry of Mines and Energy of Brazil, 2007 Brazilian Energy Balance: Executive Summary, 2007 Table 2.

Google Scholar

[2] Mart´ın C, Klinke HB and Thomsen AB, Wet oxidation as a pretreatment method for enhancing the enzymatic convertibility of sugarcane bagasse. Enzyme Microb. Technol. 2007, 40, pp.426-432.

DOI: 10.1016/j.enzmictec.2006.07.015

Google Scholar

[3] Zhao XB, Wang L and Liu DH, Effect of several factors on peracetic acid pretreatment of sugarcane bagasse for enzymatic hydrolysis. J. Chem. Technol. Biotechnol. 2007, 82, pp.1115-1121.

DOI: 10.1002/jctb.1775

Google Scholar

[4] Pandey A, Soccol CR, Nigam P, Soccol TV, Biotechnological potential of agro-industrial residues. I: sugarcane. Bioresour. Technol. 2000, 74, pp.69-80.

DOI: 10.1016/s0960-8524(99)00142-x

Google Scholar

[5] Kaar WE, Gutierrez CV, Kinoshita CM. Steam explosion of sugarcane bagasse as a pretreatment for conversion to ethanol. Biomass Bioenerg. 1998, 14, pp.277-87.

DOI: 10.1016/s0961-9534(97)10038-1

Google Scholar

[6] Kurakake M, Kisaka W, Ouchi K, Komaki T. Pretreatment with ammonia water for enzymatic hydrolysis of corn husk, bagasse and switchgrass. Appl. Biochem . Biotechnol. 2001, 90, pp.251-9.

DOI: 10.1385/abab:90:3:251

Google Scholar

[7] Swatloski RP, Spear SK, Holbrey JD and Rogers RD, Dissolution of cellulose with ionic liquids,. J. Am. Chem. Soc. 2002, 124, pp.4974-4975.

DOI: 10.1021/ja025790m

Google Scholar

[8] Dadi AP, Varanasi S, Schall CA, Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step. Biotechnol. Bioengin. 2006, 95, pp.904-910.

DOI: 10.1002/bit.21047

Google Scholar

[9] Kuo CH, Lee CK, Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments. Carbohydr. Polym. 2008, 10, pp.10-16.

Google Scholar

[10] Miller GL, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal Chem 1954, 31, pp.426-428.

DOI: 10.1021/ac60147a030

Google Scholar

[11] Rowell RM, Young RA, Rowell JK, Paper and composites from agro-based resources. Boca Raton, Fla.: Lewis Publishers, 1997, pp.86-89.

DOI: 10.1016/s0144-8617(99)00096-x

Google Scholar