Human Metallothionein Enhanced Tolerance and Biosorption of Cadmium when Expressed in Saccharomyces cerevisiae

Abstract:

The recombinant Saccharomyces cerevisiae expressing human hepatic metallothionein (MT) was constructed for biosorption of cadmium (II). The gene sequence of mt was modified for codon preference of S. cerevisiae and synthesized using chemical method. The maximal biosorption capability of cadmium compounds Cd²⁺ of the recombinant increased more than 25.8% compared with the control. For MT-expressed recombinant strains, a rapid adsorption occurred within the first 30 min with a significant level of Cd²⁺ (55.75mg/g). For comparison, S. cerevisiae cells reach its maximal Cd²⁺ adsorption capacity (45.02 mg/g) until 2h. Furthermore, recombinant strain were able to withstand the toxicity of Cd²⁺ and grow. The results indicated that recombinant should be useful in enhancement the tolerance and biosorption of cadmium in practice.

• Periodical: Advanced Materials Research (Volumes 779-780)
• Pages: 195-200
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.779-780.195
• Online since: September 2013
• Authors: Bo Liang, Min Li, Jie Shang
• Keywords: Biosorption, Metallothionein, Metals, Saccharomyces cerevisiae

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