The Effect of Oviductus Rana Hydrolysate on the Immunity Function of Low-Immunity Mice

Abstract:

Oviductus rana is a traditional medicinal product of Jilin province, China. The objective of this study was to observe the effects of the oviductus rana hydrolysate on the immune functions of immunosuppressed mice. For the purpose, this product was hydrolyzed by the combination of papain and compound proteases for which the optimum conditions of hydrolysis were also achieved. The optimum hydrolysis conditions found in this study were: composite enzyme concentration 4%; temperature 50 °C; time 4 h. The results showed that the coefficients of spleen and thymus were increased in the dose groups (P<0.05) whom oviductus rana hydrolysates were given in different concentrations as compared to the model group (without oviductus rana hydrolysate). These parameters increased as a function of concentration of oviductus rana hydrolysate. Carbon particle clearance index were also higher in the dose groups than that of model group and control. This index was the highest in the high dose group and increased as a function of dose of oviductus rana hydrolysate. The ability of macrophage as well as the delayed type hypersensitivity increased significantly in the dose groups (P<0.01). Meanwhile, the contents of IgM and IgG were also raised (P<0.01) in middle and low dose groups whereas decreased in high dose groups. It is found that oviducts rana hydrolysate had a marked effect on the immunity of immune-suppressed mice.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1248-1254
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1248
• Online since: September 2013
• Authors: Tie Hua Zhang, Yu Fei Che, Hai Yang Yan, Lu Tian, Hai Qing Ye, Xi Li, Zhuo Jun Jiang, Xin Yu Zhang
• Keywords: Cyclophosphamide, Immune Function, Oviductus Rana Hydrolysate

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