Desalination of Antioxidant Peptides from Pinctada fucata Using C18 Chromatographic Column

Abstract:

Antioxidant peptides crude product from Pinctada Fucata desalination technology by C18 chromatographic column was studied. The desalination rate and the antioxidant activity were used to analyze the effect of desalination conditions. The results show that the optimum conditions for desalting are loading 2ml of sample with concentration of 100 mg/ml, flow rate of 2 ml/min, 0.1% TFA as mobile phase with pH 6.5 in 0-4.5min and 80% methanol in 4.5-30min. Under these conditions, the desalination rate is 68.75% and the peptide recovery rate is 76.53%. The inhibitory content (IC₅₀) expressed by antioxidant peptides is 11.421mg/ml in the system of DPPH radical.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1508-1512
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1508
• Online since: September 2013
• Authors: Yan Yan Wu, Jing Wang, Lai Hao Li, Xian Qing Yang, Xiao Hu
• Keywords: Antioxidant Peptides, C18 Chromatographic Column, Desalination Technology, Pinctada fucata

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