Development of a Biotin-Avidin Mediated Immunoassay for Simultaneous Detection of Danofloxacin, Enrofloxacin and Ciprofloxacin in Milk

Abstract:

To generate a group-specific monoclonal antibody (McAb) against danofloxacin (DANO), enrofloxacin (ENR) and ciprofloxacin (CIP), glutaraldehyde was used to link the presentative hapten of CIP to the immunogen and coating antigen, respectively, leaving the common structure of these 3 drugs exposed in both conjugates as a major antigenic site. Consequently, a McAb (6D3) with high cross-reactivity to this three antibiotics has been obtained by using hybridomas technique. In a biotin-avidin mediated enzyme-linked immunosorbent assay, the IC₅₀ values for DANO, ENR and CIP were 5.1, 4.5 and 4.2 ng mL^-1, respectively. The ELISA was used for the detection of spiked DANO and ENR+CIP in milk. The recoveries ranged from 74.1 to 92.4% and coefficients of variation were in a range of 6.6-11.9%. The accuracies and sensitivity of the method were good for simultaneous analysis of the 3 drugs in milk after a simple sample extraction process.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1630-1636
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1630
• Online since: September 2013
• Authors: Jie Biao Guo, Xuan Xin, Rui Min Zhong*, Xing Ping Li
• Keywords: Ciprofloxacin, Danofloxacin, Enrofloxacin, Immunoassay, Monoclonal Antibody, Multi-Residue

References

[1] H. Ashwin, S. Stead, M. Caldow, M. Sharman, J. Stark, B.J. Keely, Anal. Chim. Acta, 637(2009) 241.

[2] C. -C. Florentina, E. -M. Anunciación, M. de la P. Arsenio, J.G. Ana, G. -G. David, Food Chem. 113 (2009) 1260.

[3] The law of the United States, Title 21 Code of Federal Regulations 530. 41(a)(10): Drugs prohibited for extralabel use in animals.

[4] Commission Regulation of the European Union No. 508/1999 of 4th March (1999).

[5] Q. Tang, T. Yang, X. Tan, J. Luo, J Agric Food Chem. 57(2009) 4535.

[6] A.L. Cinquina, P. Roberti, L. Giannetti, F. Longo, R. Draisci, A. Fagiolo, J. Chromatogr. A, 987 (2003) 221.

[7] R. Krebber, F. -J. Hoffend, F. Ruttmann, Anal. Chim. Acta, 637(2009) 208.

[8] C. González, L. Moreno, J. Small, D.G. Jones, S.F.S. Bruni, Anal. Chim. Acta, 560(2006) 227.

[9] X.M. Zhou, D. Xing, D.B. Zhu, Y.B. Tang, L. Jia, Talanta, 75(2008)1300.

[10] S. Lu, Y. Zhang, J. Liu, C. Zhao, W. Liu, R. Xi, J. Agric. Food Chem., 54(2006)6995.

[11] C. Zhao, W. Liu, H. Ling, S. Lu, Y. Zhang, J. Liu, R. Xi, J. Agric. and Food Chem., 55(2006) 6879.

[12] W. Sheng, X. Xia, K. Wei, J. Li, Q.X. Li, T. Xu, J Agric. Food Chem. 57(2009)5971.

[13] M. Jeon, I.R. Paeng, Anal. Chim. Acta, 626 (2008) 180.

[14] Y. Liu, Y. Lou, D. Xu, G. Qian, Q. ZHang, R. Wu, B. Hu, F. Liu, Microchem. J. 93(2009) 36.

[15] D. Acharya, T.K. Dhar, Anal. Chim. Acta, 630(2008)82.

[16] J. -B. Guo, Y. Xu, Z. -B. Huang, Q. -H. He, S. -W. Liu, Anal. Chim. Acta, 658(2010)197.

[17] M. Jeon, J. Kim, K. -J. Paeng, S. -W. Park, I.R. Paeng, Microchem. J., 88(2008)26.

[18] S. Bucknall, J. Silverlight, N. Coldham, L. Thorne, R. Jackman, Food Addit. Contam. 20(2003) 221.

[19] A. -C. Huet, C. Charlier, S.A. Tittlemier, G. Singh, S. Benrejeb, P. Delahaut, J. Agric. Food Chem. 54(2006) 2822.

DOI: 10.1021/jf052445i

[20] Z.H. Wang, Y. Zhu, S.Y. Ding, F.Y. He, R.C. Beier, J.C. Li, H.Y. Jiang, C.W. Feng, Y.P. Wan, S.X. Zhang, Z.P. Kai, X.L. Yang, J.Z. Shen, Anal. Chem. 79(2007)4471.

[21] Y. Zhu, L. Li, Z. Wang, Y. Chen, Z. Zhao, L. Zhu, J Agric. Food Chem. 56(2008)5469.

[22] C.M. Ju, Y. Tang, H.Y. Fan, J.D. Chen, Anal. Chim. Acta, 621(2008)200.