Determination of Chloramphenicol Residual in Freshwater Fish by HPLC

Yi Qing Yang; Qian Wang; Qiao Wang; Shi Jie Yan

doi:10.4028/www.scientific.net/AMR.781-784.1708

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Determination of Chloramphenicol Residual in...

Determination of Chloramphenicol Residual in Freshwater Fish by HPLC

Abstract:

HPLC (High Performance Liquid Chromatography) method for chloramphenicol residues in freshwater fish was developed. The samples were extracted with ethyl acetate. The LC was performed on a C18 column, mobile phase consisted of methanol-water (45:55 v/v) and eluted at 0.2mL/min at 20°C, inject volume was 20μl. The linear rang for chloramphenicol residual is within 0.5-20μg/mL, the limit of detection is 0.01ng/g. The average recovery is 80.06%-92.71%, and the RSD is 1.46%-5.61%. This method is employed to analyze freshwater fishes, such as catfish, chub and carp.

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Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

1708-1711

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.1708

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Online since:

September 2013

Authors:

Yi Qing Yang, Qian Wang, Qiao Wang, Shi Jie Yan

Keywords:

Chloramphenicol, Freshwater Fish, High Performance Liquid Chromatography (HPLC)

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